In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower
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Enzymes Reactions to Changes in Substrate and Inhibitors Benjamin J. Mora Coronado University of Texas Rio Grande Valley at Edinburgh Abstract Purpose for the experiments was to test the enzymes in various scenarios and see how changing this would affect the rate of reaction. The enzyme source used in the experiments was Turnip Extract. Concentrations of Turnip extract for activity 1 where o.5ml‚ 1.0ml‚ and 2.0 ml as for the rest of the activities 2 Through 4 stayed at a consistent concentration
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Introduction: This investigation used spectroscopy to evaluate light absorption in different solutions. A spectrophotometer was used in the lab to determine these values. A spectrophotometer is an apparatus used to “measure the absorption of radiation in the visible and UV regions of the spectrum and allows precise at a particular wave length” (Jones et al.‚ 2007). The amount of light absorbed by a substance is directly in relation to the concentration of the solute and also the wavelength moving
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Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins‚ though highly complex and diverse‚ they serve one basic function; to work as an organic catalyst. A catalyst‚ as defined by Merriam-Webster dictionary‚ is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy‚ or energy required to start a reaction. The way enzymatic reaction works cannot be altered‚ but the
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AP® BIOLOGY 2012 SCORING GUIDELINES Question 1 Note: At least 1 point must be earned from each of parts (a)‚ (b)‚ (c)‚ and (d) in order to earn a maximum score of 10. The ability to reproduce is a characteristic of life. (a) Describe the process of embryological development in a typical vertebrate embryo‚ beginning with a fertilized egg and ending with the development of three tissue layers. (4 points maximum) Embryological process Fertilization Cleavage (can occur in other stages)
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Energy and Metabolism Chapter 6 Energy and Metabolism • Organisms require the constant input of free energy and matter for growth‚ reproduction‚ and maintenance of living systems • Life requires a highly ordered system ▫ What is free energy? Energy available to do work in any system G What other types of energies do cells use? • Potential ▫ Stored energy ▫ Chemical • Kinetic ▫ Energy of motion Thermal Energy • • • • All forms of energy can be converted to heat Thermodynamics
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regular hydrogen peroxide 1%‚ 25%‚ 50%‚ 75%‚ and 100% substrate concentration tweezers timer permanent marker pen/pencil paper for data PROCEDURE: The experiment begins with five beakers full of various substrate concentrations. To begin the lab‚ gather five plastic‚ Solo cups and label them 1%‚ 25%‚ 50%‚ 75%‚ and 100% with the permanent marker. Then‚ fill each cup with 75 mL of regular hydrogen peroxide. After‚ lightly dip the filter paper into the 1% substrate concentration till it is completely
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BIOL 1364 LABORATORY EXERCISE 3 ________________________________________________________________________ INHERITANCE OF CHARACTERS Objectives of the Laboratory: i) Determination of the genetic control of seed colour‚ ie how many genes control seed colour‚ what sort of intra allelic (dominance relationship) and interallelic interactions (independent assortment vs epistasis) govern the inheritance of seed colour. ii) Understand the scientific process involved in studying character
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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concentration of substrate‚ on the activity of the enzymes. By conducting these three separate experiments also‚ three graphs are able to be obtained where the trend of each factor affecting on the enzyme activity is shown and described clearly. II. Hypothesis Experiment 1 (Effect of Temperature): As the temperature increases‚ the height of the bubble will increase too‚ indicating a faster rate of reaction. Experiment 2 (Effect of pH): Enzymes are affected by changes in pH. Extremely high or
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