MISEP Chemistry 512 – Jacobs Enzyme Catalyst Lab - Formal Report – August 8‚ 2007 ABSTRACT This investigation examined what would happen to the rate of an enzyme-catalyzed reaction if the concentration of substrate changed. We hypothesized that if the concentration increased‚ then the reaction rate would also increase. To test our question‚ we varied a combination of substrate and buffer‚ totaling 6mL‚ with a constant amount of 2 drops of catalyst. The enzyme catalyst‚ peroxidase‚ increased
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Action of enzymes as catalysts in biochemical processes * Enzymes acts as catalyst and increase the rate of all the chemical reactions. * Enzymes are also described by two properties like all other catalysts. It composed of two main functions. * The first function is that‚ they increase the rate of chemical reactions by without consumed themselves or undergo any change or alteration in the reaction. . ( Zemitec et‚al 2008). * The second function is‚ they increase reaction rates
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Enzymes All enzymes are globular proteins and round in shape They have the suffix "-ase" Intracellular enzymes are found inside the cell Extracellular enzymes act outside the cell (e.g. digestive enzymes) Enzymes are catalysts → speed up chemical reactions Reduce activation energy required to start a reaction between molecules Substrates (reactants) are converted into products Reaction may not take place in absence of enzymes (each enzyme has a specific catalytic action) Enzymes catalyse
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Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes‚ enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide‚ chicken meat‚ apple‚ carrot‚ potato‚ or chicken liver] I think that the manganese dioxide‚ apple‚ and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that
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Effect of the Changes in the Environment to the Functionality of the Enzymes Introduction a. Background In our everyday lives‚ enzymes are used in our bodies‚ and in nature around us‚ to speed up the chemical reactions happening constantly‚ which happens by lowering the amount of activation energy needed to start various reactions. The way this works is by attaching the particular substrate to the active site of the enzyme‚ where it will start to aid the chemical reaction. Then‚ the allosteric
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Enzyme Report Case 1 - Hereditary Fructose Intolerance 1 & 2. Enzymes take on a variety of roles in the human body at the cellular level. Specifically‚ they aid in the breakdown of macronutrients such as glucose and fructose so that the body can use them. Although reactions within the body would occur without enzymes‚ enzymes provide control because the reaction is not needed all of the time. Enzymes function in specific environments such as temperature or pH; some enzymes are specific
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TASK 4: Explain the structure of the ileum and the ultrastructure of the epithelium in relation to its secretory and absorptive functions. (a) Explain the characteristics of enzymes. (b) Explain the lock and key theory of enzyme action. (c) Locate and explain the role of important digestive enzymes. TASK 5: Enzymes are involved in anabolic and catabolic reactions: a) Define the terms anabolic and catabolic reactions b) Give 3 examples of anabolic and 3 examples of catabolic reactions
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Bio Lab Report In part II of the Organic Compounds in Foods lab‚ we to examining whether or not the starch in the saltine cracker can chemically break down in sugar with the presence of salivary amylase‚ which is an enzyme found in your mouth which assists in digestion. My group and I were instructed to obtain a saltine cracker from our teacher and chew on it for approximately 2 minutes. After concluding the chewing‚ we then grabbed a beaker and spit our slimy and chewed cracker into it. Subsequently
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The reactions and Results of Using Enzyme Turnip Root Peroxidase Lab results from: Andrew Compton‚ Mickey. Results published 9/29/2012. TA: In this series of laboratory experiments‚ my lab partner and I were to conduct an experiment about the oxidation rate of the enzyme peroxidase in the presence of its substrate guiacol. Also we used other substrates‚ such as hydroxylamine an enzyme inhibitor‚ to observe the weather the reaction rate was slowed down‚ sped up‚ or stopped reactions all together
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