Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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KOLEJ MATRIKULASI MELAKA 78300 MASJID TANAH‚ MELAKA Guidelines for Biology Experiments 1.0 Attendance 1.1 Attendance to practical sessions is COMPULSORY. 1.2 If you cannot come to the class due to ill health or emergency excuses‚ please inform the lecturer EARLIER‚ so that another practical session can be arranged for you ON THE SAME WEEK. 2.0 Lab coat 2.1 Wearing lab coat is COMPULSORY. 2.2 Put on the lab coat throughout the class. 3.0 Jotter 3.1 Jotters should contain the summary on the PROCEDURES
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Objective: To find out the food substance in food sample X‚Y and Z. Biological Principle: Test for lipids using the grease spot test Test for glucose using Clinistix paper Test for reducing sugars using Benedict’s test Test for starch using the iodine test Test for proteins using Albustix paper Test for vitamin C using DCPIP solution A control using distilled water instead of the sample should be included. Independent: different types of food samples Dependent: different types of food
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Lab Report: Flowers‚ Fruits‚ and Seeds Part 1: Flowers Directions: Answer the questions below using the picture of the Gladiolus‚ or Lily flower‚ below. 1. How many petals does this flower have? From this point of view‚ I can see 10 colorful petals. 2. 3. How many stamens does this flower have? I see about 3. 4. 5. How many stigmas does this flower have? I can see about 6 or 7 stigmas. 6. 7. Is this the flower of a monocot or a dicot? A monocot. 8. 9. Explain your answer for question
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Chapter 20: DNA Technology Biotechnology: Use of living organisms to perform tasks. * Wine & cheese * Selective breeding * Antibiotic production * Recombinant DNA Restriction Enzymes * Bacterial enzymes: cut up foreign DNA * Specific: only but at recognition sequences * Palindromic: cut at the same base sequence on each strand‚ but in the opposite direction * The exposed bases provide “sticky ends” * H-bond to compliment bases of segments cut with same restriction
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28/09/12 Grace Masters Woodlice Behavior Experiment Aim: To investigate the effect of light intensity on the behavior of woodlice (Porcellio scaber) in regards to their change in speed. Variables: Variable How can it be controlled? (IV) - Light intensity - The light intensity will be controlled by the knob on the lamp throughout the experiment. In addition‚ the light intensity will be controlled by closing the windows and doors in the classroom as well as turning
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1. By exhaling out carbon dioxide into the straw on top of the bromothymol blue indicator‚ the solution will turn the color blue into a yellowish-green color. When there is a presence of something that is acidic the BTB indicator changes its color into yellow/green. So in this case‚ the water with BTB and carbon dioxide exhaled into the flask creates a carbonic acid. As an aerobic activity increase than the carbon dioxide rate increases as well. 2. By exercising more‚ one’s body requires more
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Iodine is a test for starch while Benedict’s solution is a test for reducing sugars. When solution A is tested by benidicts test‚ the clear blue solution changed to a little reddish and brick red precipitate is formed.this result show that solution A is a reducing sugar. When carried out iodine test with solution A‚ the colourless solution remain unchanged . this tell us that starch is absent is solution A. When solution B is tested with Benedicts test‚ the clear blue solution remain unchanged‚ we
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Introduction: Living‚ microscopic‚ unicellular organisms can be identified as microorganisms. Fungi‚ Protista‚ Bacteria‚ and Archaeans are four kingdoms in the phylogenetic tree of life that consist of microorganisms. Amongst these microorganisms the prokaryotes were the first ones to arrive through evolution. The rest of the life forms as we see them evolved from these simple creatures‚ prokaryotes. The smaller prokaryotes were swallowed by bigger prokaryotes setting the stage for the origin of
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11 ST Revision i) Multiply the following numbers and express in terms of a x 10k where 1 ≤a ≤ 10 and k ϵ Z: 3.2 x 104 5.6 x 10-2 ii) Add them and multiply by four. iii) Factorise x2 – 49 iv) Factorise 16x2 - 9 v) Factorise x2 – 7x + 12 vi) Solve x2 -7x + 12 = 0 vii) The first term of an arithmetic sequence is 8 and the common difference is 7. The nth term is 393. Find the value of n. viii) A geometric sequence has first term 2 and third term 32. Find the common Ratio. ix) Find
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