3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
Premium DNA Protein Molecular biology
Fast Plants Lab Background Gregor Mendel is an Australian monk‚ who worked in a garden at a monastery. He experimented with pea plants‚ and soon became known as the father of genetics. The reason he studied pea plants was because they grow quickly‚ the traits can be easily observed‚ and the plant is easy to pollinate. Mendel’s method was to control the pollination of the pea plants and create offspring’s of the two plants that were pollinated together. Using self-pollination and cross pollination
Premium Gregor Mendel Genetics Gene
11 ST Revision i) Multiply the following numbers and express in terms of a x 10k where 1 ≤a ≤ 10 and k ϵ Z: 3.2 x 104 5.6 x 10-2 ii) Add them and multiply by four. iii) Factorise x2 – 49 iv) Factorise 16x2 - 9 v) Factorise x2 – 7x + 12 vi) Solve x2 -7x + 12 = 0 vii) The first term of an arithmetic sequence is 8 and the common difference is 7. The nth term is 393. Find the value of n. viii) A geometric sequence has first term 2 and third term 32. Find the common Ratio. ix) Find
Premium Venn diagram Geometric progression Integers
Online Biology Lab Report Evolution Name______ A. Natural Selection 1. Record the number of beetles present in each of the three generations from the lab website. Generation|Orange Beetles|Green Beetles| 1st Generation|3|13| Later Generation|6|10| Last Generation|16|0| 2. Nature selected for the _______orange__________ beetles. 3. Nature selected against the______green________ beetles. B. Speciation 1. Why were wasp species A and B no longer able to mate with each
Premium Animal Larva Evolution
How does photosynthesis model the Law of Conservation of Matter and The Law of Conservation of Energy? Photosynthesis models the Law of Conservation of Matter and the Law of Conservation of Energy‚ and there is evidence from some articles that were read to prove this statement. First‚ the Law of Conservation of Matter is modeled in photosynthesis‚ because in the Photosynthesis for Kids article it stated‚”Photosynthesis occurs when Chlorophyll‚ a green pigment that collects lights‚ consumes radiant
Premium Energy Chemistry Oxygen
➢ Competing – Two species compete with another species for resources such as food‚ water‚ or territory ➢ Biomes – major types of ecosystems that occupy very broad geographic regions. ➢ Photic zone is where there is enough light for photosynthesis to occur and an aphotic zone‚ where very little light penetrates. ➢ Benthic zone - is located at the bottom of the biome ➢ Oligotrophic lakes are deep lakes that are nutrient-poor and oxygen-rich and contain sparse phytoplankton.
Premium Ecology Trophic level Food chain
Prokaryotic. Example: Salmonella‚ peptidoglycan makes up a cell wall type thing Archaea: Most are unicellular and microscopic. Prokaryotic. Example: ancient bacteria‚ live in harsh enviorments‚ Eukarya: Contain a nucleus and organelles. Eukaryotic. Example: Plants‚ animals‚ fungi and all other forms of life -All have cells‚ dna‚ rna‚ membrane‚ ribosomes Producer to Tertiary Consumer in an energy pyramid (trophic levels) -Trophic structure / levels~ feeding relationships in an ecosystem -Primary producers~
Premium DNA DNA replication
Photosynthesis Lab Answer Key Vocabulary: carbon dioxide‚ chlorophyll‚ glucose‚ limiting factor‚ nanometer‚ photosynthesis‚ wavelength Prior Knowledge Questions (Do these BEFORE using the Gizmo.) [Note: The purpose of these questions is to activate prior knowledge and get students thinking. Students are not expected to know the answers to the Prior Knowledge Questions.] • To survive‚ what gas do we need to breathe in? Oxygen • Where is this gas produced? In plants Gizmo
Free Light Carbon dioxide
Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
Premium Cell culture Cell
metaphase ll the chromosomes align on the metaphase plate in each cell. The process of anaphase ll pulls the sister chromosomes to opposite poles in each cell. During telophase ll each cell forms a nuclei and cytokinesis splits the cells apart. Four genetically different haploid cells are produced. Independent assortment is differences between the genes. Variation can occur in meiosis when the chromosomes cross over and during random fertilization. Random fertilization is when a gamete from
Premium Mitosis Meiosis Chromosome