Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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Chapter 1 Exploring Life Lecture Outline Overview: Biology’s Most Exciting Era Biology is the scientific study of life. You are starting your study of biology during its most exciting era. The largest and best-equipped community of scientists in history is beginning to solve problems that once seemed unsolvable. Biology is an ongoing inquiry about the nature of life. Biologists are moving closer to understanding: How a single cell develops into an adult animal or plant. How plants convert
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Andy Ton Ms. Morrissey AP Biology 9 January 2012 Fish Classification Lab |Fish # |Key # |Fish Name | |1 |1b>12b->13b->16a |Atlantic Salmon | |2 |1b->12b->13a->14b |Bullhead Catfish | |3 |1a->2b->6b->8b->10b |Blue Gill | |4 |1a->2a->3b->5b
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AP ESSAY ANSWERS: 16-20 1. Information transfer is fundamental to all living organisms. For TWO of the following examples‚ explain in detail‚ how the transfer of information is accomplished. A) The genetic material in one eukaryotic cell is copied and distributed to two identical daughter cells. B) A gene in a eukaryotic cell is transcribed and translated to produce a protein. C) The genetic material from one bacterial cell enters another via transformation‚ transduction or conjugation
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genetic information and copies itself (replication) to pass on the information • Contains genes (instructions to make proteins) • Instructs cell’s activities DNA Structure • DNA is a polymer of nucleotides • Chromosomes (DNA strand + associated proteins ie. Histones wrap DNA around like a spool = condensed chromatin) ↓ genes (sections of a chromosome that codes for a protein) ↓ nucleotides (3 parts: phosphate‚ deoxyribose sugar‚ and nitrogen base) • • • Double helix shape - Held together
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The Role of DNA in Protein Synthesis DNA is found in the cells nucleus. It is part of the chromosome of the cell. DNA is the molecule that controls the making of protein. Protein is used for the growth of cells and organisms and also for the repair of cells. Protein is also used as an enzyme to speed up reactions in the body. Because of this DNA is able to control the whole organism. With out DNA and its ability to make protein the cells and eventually the whole organism would die
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in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe that both types of treated cells will express the beta-actin gene because it is a housekeeping gene. However‚ only HL-60 cells treated with PMA will express the MMP-9 gene by their RNA. The purpose of this lab is to view the effects of differentiation on protein synthesis
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lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used included lysozyme‚ protamine sulfate‚ and bovine serum albumin‚ and the three non-protein samples contained either RNA‚ tyrosine‚ and glycylglycylglycine. Standard curves were created to exhibit the linear relationship between the concentration of solute (protein‚ non-protein)
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Produced by cell clusters in the pancreas called the Islets of Langerhans‚ insulin is a hormone that regulates glucose concentration in the blood. It is a protein synthesised and stored by beta cells‚ which secrete it in surges responding to changes in glucose levels. (Hardin‚ Bertoni and Kleinsmith‚ 2012) Fig 1. The Pancreas and Insulin |Source: University of Maryland (2011) | | |
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AP biology FRQ Courtney DeMane 1.22.13 block 7 A). The chemical composition and structure of proteins seems quite confusing at first but one it is broken down into levels it is much more understandable. Amino acids are the basic building blocks of proteins and they contain amino‚ carboxyl and R groups. These R groups that are in the amino acids are what determine the properties of the specific amino acids. For structure‚ there are 4 levels. The first one being the primary level. These are made
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