corresponding anthocyanidins using paper chromatography‚ and spectrophotometric analysis procedures. Based on experimental data the blueberries contained the most amount of anthocyanins at 1.92 x 10-6 g‚ followed by blackberries at 1.19 x 10-6 g‚ finally followed by raspberries at 5.84 x 10-7 g. The paper chromatography data also supports the idea that blueberries contained the most anthocyanidins‚ since it contained the most streaks on the chromatography paper. The main conclusion that can be
Premium Cell Oxygen Chemistry
Plant Pigment Chromatography VII. Analysis (Questions) 1. What factors are involved in the separation of pigments? Factors that are involved in the separation of pigments can include pigment solubility‚ the attraction between the pigments and paper and the size of each pigment particle. Because of these factors the results were as they were. Beta-carotene traveled the furthest because it forms no hydrogen bonds to the chromatography paper and is slightly soluble in the solvent. Contrastingly
Premium Chemistry Solubility Solvent
What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
Premium Chromatography Analytical chemistry Gas chromatography
CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
Premium Photosynthesis Solvent Leaf
Liquids & Solids Pre Lab Questions: 3pts 1. What are the learning goals of this lab? The learning goal of this experiment is to view and experience the melting point and boiling point of different substances. 2. Why is acetamide not allowed in contact with your skin? What precautions does one have to take to work with this chemical? Acetamide is a carcinogen‚ which can cause cancer. Therefore‚ it should not be allowed to come in contact with a person’s skin. Any person working with this chemical
Premium Temperature Liquid Fahrenheit
Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. There are several kinds of distillation methods. However‚ the method that we used in this experiment was fractional distillation. This method is used when trying to separate two different volatile compounds whose boiling points differ by 40-50°C or more. If the boiling points are too close‚ this method
Premium Distillation Evaporation Water
Component seeks to separate‚ identify‚ quantify and analyze the active component found in Biogesic®‚ which may be useful for public awareness despite its wide acceptance and availability in the market. Furthermore‚ this study applies thin layer chromatography (TLC) technique since it is the simplest‚ cheapest yet most effective approach in terms to the study’s objectives. The TLC method implies Rf (Rate of flow) values where it is given by the distance from origin to center of subtance spot over the
Premium Thin layer chromatography Chromatography Chemical polarity
Rate of Evaporation of Different Liquids Objective of the Project This project is of the rate of evaporation of different liquid‚ in which we also discuss the factors which affect the rate of liquid. Introduction When liquid is placed in an open vessel. It slowly escapes into gaseous phase ventually leaving the vessel empty. This phenomenon is known as vaporization or evaporation. Evaporation of liquids can be explained in the terms of kinetic molecular model although there
Premium Liquid Kinetic energy Boiling point
Calculating the Density of Liquids II. Purpose/Background: The purpose if this lab was to determine the density of water and an unknown liquid. Density is defined as the mass of a substance divided by its volume. It is an intrinsive property of matter and is used to specifically characterize substances. III. Procedure: 1.We massed an empty 10mL graduated cylinder to the nearest .01g. 2.Then‚ we filled the graduated cylinder with 4.0-5.0 mL of distilled water‚ 3.massed it to the nearest 0.1
Free Density Water
Thin Layer Chromatography/ Paper Chromatography Lab # 10 11/26 Sample # 32 for TLC Sample # 1 for Paper Introduction: Chromatography is one of the most important separation techniques used in all fields of chemistry ranging from analytical chemists to pharmacists. The understanding of how chromatography works and how to operate instruments used to carry out the procedures is an important lab technique to learn. Experiment: Thin Layer Chromatography. Lab #10-1 Paper Chromatography
Premium Thin layer chromatography Chromatography