Experiment 2: Fractional Distillation and Gas Chromatography Introduction: The purpose of this experiment was to separate a mixture of ethyl acetate (EtOAc) and toluene by using the method of fractional distillation and to analyze the fractions using gas chromatography (GC). ethyl acetate toluene Distillation is a method known as separating or purifying a liquid or mixture by vaporization and condensation. In a simple distillation‚ the liquids being separated boil below 150 0C at 1 atmosphere
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CLINICAL CHEMISTRY – defined as the study of biochemical processes associated with health & dse & the measurement of constituents in body fluids or tissues to facilitate dx of dse - dual nature since it’s both a basic and applied science thus clin chem. technologists & technicians should have an understanding of the physiologic & biochemical processes occurring in the body‚ as well as technical skills to perform the various tests - chemistry in connection with the management
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Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase‚ which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds‚ causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound’s partition coefficient result in differential retention
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1 in vapour state sulphur partly exists as S2 molecule which has 2 unpaired electrons in the antibondind pi orbitals jus lyk oxygen and thus exhibits paramagnetism. 2 An interhalogen compound is a molecule whose atom contains two or more different halogen atoms (fluorine‚ chlorine‚ bromine‚ iodine or astatine). Most interhalogen compounds known are binary (composed of only two distinct elements). Their formulas are generally XYn‚ where n = 1‚ 3‚ 5 or 7‚ and X is the less electronegative
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Gas Chromatography Analysis of Product Mixtures Gas Chromatography Guidelines‚ Handout. Introduction Gas chromatography is a technique used to analyze chemical compounds that can be vaporized and separated in a gas phase column. Once separated‚ the analyzed substance is passed through a detector and data is obtained. The samples that we are going to analyze are: the EtOAc from Simple distillation‚ the Fraction 1‚ Fraction 2‚ and Fraction 3 from the Fractional Distillation. Experimental Procedure
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The mobile phase can be liquid‚ gas‚ or supercritical fluid. How Does Chromatography Work? Chromatography is a surface effect. It works by first starting off with an unidentified liquid being injected into a column. As the chemicals in the liquid move down the column‚ they separate depending on their physical and chemical attributes‚ such as speed and weight. To easily picture the process‚ the example will pertain to a paper chromatography process in which the liquid is moving over the paper‚
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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CHAPTER # 1 Q.DEFINE CHEMISTRY. A. Chemistry‚ a branch of physical science‚ is the study of the composition‚ properties and behavior of matter. Chemistry is concerned with atoms and their interactions with other atoms‚ and particularly with the properties of chemical bonds. Chemistry is also concerned with the interactions between atoms and various forms of energy Q.DEFINE THE BRANCHES OF CHEMISTRY. * Physical chemistry is the study of macroscopic‚ atomic‚ subatomic‚ and particulate phenomena
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A Homemade Dishwashing Liquid A Scientific Research Project Presented to the Research Department College of Engineering‚ Technology and Computer Science ___________ In Partial Fulfillment of the Requirements for the Course General Chemistry ____________ Presented by: Bañares‚ Michael Ray Romero‚ Jo Cris Fernando‚ Caroline Ebora‚ Mark Bryan Escuro‚ Edmund BSIT Electrical A11AM October 2013 Republic of the Philippines City of Taguig Taguig City University
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Diffusion is the movement of molecules from areas of high concentration to areas of low concentration. With diffusion no energy is required and it is caused by kinetic energy. The rate of diffusion can be affected by many things including temperature‚ molecular weight‚ and movement. To test the rate of diffusion a drop of dark food coloring was added to a glass of tap water and it was timed to see how long it took for the drop to reach the bottom of the glass. This procedure was repeated three
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