BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Abstract: This lab tests how temperature and pH affect how enzymes will function. The lab showed that temperature will denature an enzyme when past its optimal working temperature and won’t denature in cold temperatures‚ but have slowed molecular activity. pH will also have an affect on an enzymes efficiency‚ when out of optimal pH the enzyme will not function as it is supposed to and if to far out of the optimal pH the enzyme will change shape and no longer work. Enzymes also showed to be reusable
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AP Biology notes: Chapter 2- The Chemical Context of Life 1. Overview * The bombardier beetle fights the ants that plague it by using chemistry. * The natural systems and environment that organisms live in involves chemistry and physics. * Biology is not the only category of science. There is also chemistry‚ physics‚ and engineering. * Organization of life is separated into a hierarchy of structural levels. 2. Elements and Compounds * Matter- anything that takes up
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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Study Guide for Unit V Energy and Enzymes. What is cellular work? Why must living things utilize energy with maximum efficiency? Define energy. Why do living things need a constant input of energy? Where does all energy in the biosphere originate? Define kinetic and potential energy. (Give biological examples). What are the two laws of thermodynamics? Give examples. What is entropy and what is the law of entropy? How do living things resist the law of entropy? Give biological examples. Define
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------------------------------------------------- Module Two: Lab Questions Your first lab link can be found at Five Dangerous Things for Kids A written transcript is also available for this lab. 1. Do you agree with Gever Tulley that the increasing safety regulations for children are limiting children’s opportunities to interact with the world around them? Why or why not? Yes‚ I agree with Gever Tulley that increasing safety regulations for children are limiting children’s opportunities to
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Virtual Lab: Enzyme Controlled Reactions Worksheet 1. Which of the following does NOT apply to an enzyme: b. Inorganic 2. When an enzyme catalyzes a reaction: a. Substrate(s) bind in the active site 3. Which of the following would interfere most with the ability of an enzyme to catalyze a reaction? a. Reduced concentration of substrate available 4. Feedback mechanisms regulate the rate of enzyme activity‚ effectively “turning off” an enzyme in a reversible way until more product is needed
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Chemistry Unit 1 – Revision Questions Chemistry 1A 1) Define an element. How many are there in the Periodic Table? 2) Write a table with the charges and masses of protons‚ neutrons and electrons. 3) Define atomic number and mass number. 4) Draw electron (energy level) diagrams for Be‚ S‚ Al‚ Cl and K. Also‚ write down the number of protons and neutrons for each. Finally‚ write the symbols (with mass no. and atomic no.) 5) Why have all Group 7 elements (halogens) got similar chemical properties
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Enzyme Kinetics Marcos‚ Nelissa S. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ABSTRACT The rationale of the experiment is basically founded in the concept of reaction rates as affected by enzyme‚ and how the enzyme works is competed by a competitive inhibitor‚ thereby impeding the forward reaction. In this experiment‚ o-diphenol oxidase‚ an enzyme that causes the browning in fruits‚ was extracted from banana and reaction rate of this was established
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showing instructor.pub; ISSC362 Week 1 Lab #2: Implement Hashing and Encryption for Secure Communications Instructor Name: ________________ Lab Assessment Questions 1. Compare the hash values calculated for example.txt that you documented during this lab. Explain in your own words why the hash values will change when the data is modified. The Hash Value is set to a exactly the value so once it is altered the values will change to the alteration. 2. Why are the MD5sum and SHA1sum hash values
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