reactions Keywords: Alkaline phosphatase; kinetics; Enzyme-cofactor interaction; synergism * corresponding author. Email: femijohn@gmail.com 43 INTRODUCTION The roles of metal ions in metalloenzymes include direct participation in catalysis‚ stabilization of protein structure and regulation of enzymatic activity. Membrane alkaline phosphatase (ALP) is a metal-containing enzyme that serves as a good model for the study of metal ion interactions in enzyme catalysis. Native E. coli ALP contains three
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Reactions Enzymes are proteins found in living things that speed up chemical reactions. They aid in nearly all metabolic processes‚ such as food digestion‚ molecule synthesis‚ and the storage/ release of energy. An enzyme speeds up the rate of the chemical reactions by lowering the reaction’s activation energy‚ which means that by definition‚ an enzyme functions as biological catalyst. The activation energy is the energy that is used to get a reaction started. The function of an enzyme is dependent
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Enzymes Enzymes are… * Biological catalysts Lower the energy level needed for a biochemical reaction to occur. This energy level is called activation energy. * Proteins Polypeptide chains made up of 100’s-1000’s of amino acids in a specific sequence. * Do not get “used up” in a reaction The number of “uses” of an enzyme depends on the enzyme. * Work more efficiently at certain optimum temperatures. * They are “reaction-specific”. Each enzyme is included in one reaction.
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__________________________________ AP Biology LAB 2. ENZYME CATALYSIS ESSAY 2000 The effects of pH and temperature were studied for an enzyme-catalyzed reaction. The following results were obtained. a. How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer‚ include a discussion of the relationship between the structure and the function of this enzyme‚ as well as a discussion of how structure and function of enzymes are affected by temperature and pH. b. Describe
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Procedures for Part A: For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled
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syrup (referred to as Caliber)2. Beet juice is the by-product in the production molasses for sugar beets.3 The by-product is generally not processed further (depends on manufacturer) and commonly contains minute particles dispersed through-out liquid. As a result‚ beet juice based blends have a greater tendency to clog filters vs. caliber based blends.1 Performance Characteristics: Caliber possesses a significantly better eutectic point than that of beet juice. This translates to a greater melting
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Jamba Juice is a chain of smoothie restaurants headquartered in Emeryville‚ California with over 700 locations operating in 30 states‚ the District of Columbia and the Bahamas. Over 500 locations are company-owned‚ with the remainder being franchised.[1] For the 51-week Fiscal Year‚ which ended on January 1‚ 2008‚ sales were $317 million‚ which included sales of $306 million from company locations. Jamba Juice was founded by a Crespi student in April 1990 by Cal Poly graduate Kirk Perron‚ along
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Jamba Juice Case Study 1/3/14 GBA 513 James Albright Patrick Carson Elizabeth Forte Laura Griffin Introduction Founded in April of 1990 by Cal Poly graduate Kirk Perron‚ Juice Club‚ Inc. opened its first store in San Luis Obispo‚ California. In 1993‚ Juice Club opened and started two other stores‚ one in southern California and one in northern California. Juice Club Inc‚ changed its name to Jamba Juice in 1995 and became known as one of the leaders in healthy juices and smoothies
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Measures of Success 14. Recommendations 15. Conclusion Background Boost Juice Bars (Boost‚ 2014) is an Australian food and beverage brand‚ which was formed in 2000 with the first store located in the capital city in the South part of Australia- Adelaide. This food and beverage business has been done well since the business has been expanded globally in Asia‚ Europe‚ and Russia and even in Middle East. Unfortunately‚ Boost Juice Bars stopped part of business in New Zealand in 2006 after the franchiser
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Jamba Juice SWOT Analysis is a basic model that assesses the business environment of an individual firm. This tool identifies the strengths‚ weaknesses‚ opportunities and threats of an organization. An overview of the four factors in this case study is given below 1. Strengths • Jamba Juice is a well-known company and this company grew rapidly to become a top brand in the industry. • This organization has a unique company culture. • This company provides many options and menus of healthy products
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