The control plates used in transformation are the LB and second LB/Amp plates marked with a “-“. The control for restriction digest was the ladder. The independent variables are the different cultures of the E. coli‚ and the +LB/Amp and +LB/Amp/ara plates form the experiment group. The dependent variable is whether the cells glow green under the UV light and whether they are resistant to ampicillin or not. The variables in restriction digest are the other 4 samples. The dependent variable is the
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2. There is no one best way to make change in an organisation. How does this inform your understanding of organisational change towards greater sustainability? Discuss Introduction Organisations are faced with new changes constantly. In order to remain successful and respected‚ it is important that they respond to changing situations appropriately (Waddel‚ Cummings and Worley‚ C 2011). There are many factors that influence an organisation to change; the one that this piece will review is sustainability
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Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia
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Hara (KARA) * Gain (Brown Eyed Girls) ------------------------------------------------- H[edit] * Han Seungyeon (KARA) * Ham Eun-jeong (T-ara) * Han Sunhwa (SECRET) * Ha Ji Won * Haha * Heo Ga Yoon (4Minute) * Heo Young Saeng (SS501) * Hong Jin-Young * Hong Kyung Min * Hyolyn (Sistar) * Hyomin (T-ara) * Hyun Bin * Huh Gak * Hwangbo (Chakra) * Hwang Chansung (2PM) * Tiffany Hwang (Girls’ Generation) * Hwanhee * Hwayobi
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The Principate: The Aeneid as Augustan Propaganda The ascension of Augustus to supreme power in 31 BC signaled a distinctive break in Roman political history‚ from republic to monarchy‚ albeit veiled in apparent conservatism. The creation of an official Julii mythology served to legitimize Augustus and his dynasty; on the insistence of Augustus‚ Virgil wrote the Aeneid to demonstrate the mythological foundations of the Julii line‚ and how the future of Rome‚ and consequently the reign of Augustus
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Katherine Ms. Collins 11IB English B 2 April 2015 Summary: Treatment of Maori Intolerance towards indigenous people is typical colonial societies. European colonists settled in New Zealand justified their exploitation and enslavement as culturing and civilizing native people which were regarded as uncivilized people by European colonists (mainly British people). In New Zealand‚ Māori‚ as individuals and communities‚ were the subject of racism and discrimination when Europeans immigrants (Pakeha)
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Introduction Bacterial transformation is the permanent alteration of a bacterial cell genotype as a result of its uptake and incorporation of foreign DNA fragments from external medium (Anthony et al‚ 2008). In addition to chromosome‚ bacterial cells often contain extrachromosomal DNA called plasmids which are capable of autonomic replication and antibiotic resistance (Dale & Simon‚ 2010). Plasmids can transport foreign DNA into host or other bacterial cells hence they are known as vectors.
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Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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is a voluntary compliance rather than a mandatory one and that the authenticity of a company’s true social impact is establishing itself....... and that these companies will employ socially responsible activity without any regulatory requirements. (Aras & Crowther‚ 2008‚ p. 10) It will be discussed whether companies will have to become more responsible for their affect on the external environment with mandatory compliance and regulation or whether it simply will make no difference. There is a belief
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Difference in Memory MAHUA SARKAR Sociology‚ Binghamton University‚ SUNY Everyone lives in a story . . . because stories are all there is to live in‚ it was just a question of which one you chose . . . (Amitav Ghosh‚ The Shadow Lines)1 INTRODUCTION The study of popular memory is necessarily relational. It involves the exploration of two sets of relations: (1) that between dominant memory and oppositional forms across the public field‚ including academic productions; and (2) the relation between
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