Genetic Engineering Biology and Society: Crime Scene Investigations: Murders in a Small Town On November 22‚1983 - A 15-year-old girl was raped and murdered on a quiet country lane. - Three years later‚ another 15-year-old girl was raped and murdered. DNA fingerprinting of DNA samples from suspects and the crime scene - Proved one man guilty and another man innocent. Recombinant DNA Technology Recombinant DNA technology is a set of techniques for combining genes from different sources
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Study Questions: Chapter 28 1.) Archaea and Bacteria are the oldest‚ structurally simplest and most abundant forms of life. 2.) Name and describe seven ways in which prokaryotes differ substantially from eukaryotes? Unicellularity- fundamentally single-celled Cell Size- can vary (large range changes with species) Nucleoid- lack a membrane-bound nucleus but rather a nucleoid region Cell Division/Genetic Recombination- binary fission (does not use spindle) and do not have a sexual cycle Internal
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Chapter 1 Humans and the Microbial World A Glimpse of History Science of microbiology born in 1674 Antony van Leeuwenhoek (1632–1723) • Made simple magnifying glass • Studied lake water • Observed ‘animalcules’ Robert Hooke • Also credited with discovery • Described ‘microscopical mushroom’ (common bread mold) in 1665 1 Importance of Microorganisms Microorganisms are foundation for all life on earth Have existed for ~3.5 billion years Plants‚ animals‚ modern
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A virus is a microscopic agent‚ which is not cellular (acellular) and is composed of nucleic acid surrounded by a protein coat. ● The germ theory of disease‚ first proposed in the 1800s stated that microorganisms could cause infection and illness. ● Archaea are prokaryotic singlecalled organisms of primitive origin that are capable of living in extreme or very harsh environment and are classified in their own domain of life. ● Nomenclature is the term applied to the method of assigning a scientific or specific
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chm.msu.edu/vibl/content/gramstain.html b. Click “Start” and allow animation to run. c. Click “View Slide under the Microscope” d. Click “Examine Samples” e. Complete the table 4) Research: Select either one group or a specific species within the Archaea and discuss their relevance to humans. Analysis / Data: Activity Page 1 Present in all cells Present only in (some or all) prokaryotes Present only in (some or all) eukaryotes DNA X Nucleus X Ribosomes X Cell membrane X Flagella made
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Among them‚ the primer sets GM3F / GM4R and EUB968F / UNIV1392R were used in PCR for bacteria while the primer set ARCH622F / ARCH934R was PCR applied for archaea. All PCR amplifications were carried out in 30 pi of a pH 8.3 buffer (Pharmacia Biotech‚ Piscataway‚ NJ) containing 200 pM each of the four deoxynucleotide triphosphates‚ 15 mM MgCl2‚ 0.1 pM of each primers‚ and 1 unit Taq polymerase (Pharmacia Biotech
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Chapter 1 Introduction: Themes in the Study of Life PowerPoint® Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero‚ updated by Erin Barley with contributions from Joan Sharp Copyright © 2008 Pearson Education‚ Inc.‚ publishing as Pearson Benjamin Cummings Overview: Inquiring About the World of Life • Evolution • Biology Response Evolutionary to the environment adaptation • Life is recognized by what livings things do
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pro=before karyon=kernel or nucleus eu=true karyon=kernel or nucleus Domains of Bacteria 1. archaea or archaebacteria‚ more ancient in soil and water also in hostile environments‚ many without oxygen high salt/brine methanogens halophiles thermoacidophiles hot acidic springs 2. bacteria or eubacteria‚ more recent‚ very different biochemistry from archaea regular bacteria in soil‚ water‚ pathogens cyanobacteria Animal Plant Fungi Protist
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chap 17: 1) Which of the following variations on translation would be most disadvantageous for a cell? A) translating polypeptides directly from DNA B) using fewer kinds of tRNA C) having only one stop codon D) lengthening the half-life of mRNA E) having a second codon (besides AUG) as a start codon a 5) A particular triplet of bases in the template strand of DNA is 5’ AGT 3’. The corresponding codon for the mRNA transcribed is A) 3’ UCA 5’. B) 3’ UGA 5’. C) 5’ TCA 3’. D) 3’ ACU 5’. E) either UCA
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1: The Microbial World and You Learning Objectives Go Over First Lecture 1-1 List several ways in which microbes affect our lives. 1-2 Recognize the system of scientific nomenclature that uses two names: a genus and a specific epithet. 1-3 Differentiate the major characteristics of each group of microorganisms. 1-4 List the three domains. 1-5 Explain the importance of observations made by Hooke and van Leeuwenhoek. 1-6 Compare spontaneous generation and biogenesis. 1-7 Identify
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