EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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result in Benedict’s test as the colour of solution change from blue to brick-red and there is brick-red precipitate. Starch give positive result in Iodine test as the colour of solution change from yellow to dark blue. Glucose is the simplest carbohydrates that are monosaccharide or simple sugar. Starch consisting of a large number of glucose units joined together by glycosidic linkage and known as polysaccharide‚ that is polymers of sugar. Saliva that contains salivary amylase enzyme‚ which breaks
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In the Middle East‚ it is extremely common for parents to arrange a marriage.This is precisely what occurs to Anilia and Samia and it will forever change their lives. In “Same Story Different Ending” by Anila Batool‚ a girl from Pakistan meets a boy in college and starts to question her parents and religion. Whereas‚ in “Another Even at the Club” by Alifa Rifaat‚ a 16 year old still in high school is married to an older man by there parents. And now Samia is being controlled by her husband. Anila
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Souvenir 2013 Optical Fiber Project & its Impact Background In the initial days‚ the backhaul of telecom equipments relied on microwave and satellite communication. The rapid proliferation of various telecom technologies and the desire to penetrate larger market segments resulted in high national and international traffic. The high cost of space segments for statellite communications and limited capacity of Microwave links compelled Nepal Telecom to embrace the optical fiber deployment to cater high
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1 diabetes and is very physically active. An example of a healthy day of eating for Jason would be three meals that do not individually exceed 60 grams of carbohydrates. As for between meals and to promote energy for sports‚ it would be a good idea for Jason to choose nutrient dense snacks that do not contain more than 30 grams of carbohydrates. Some snack suggestions might be a small granola bar‚ nuts and fruit‚ or peanut butter crackers. Jason’s morning meal could be a breakfast burrito that includes
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There was a lot of rich literary material written by Maxine Kingston in China men but I think On Mortality is one of the most interesting stories I have ever read because it’s so short‚ yet so adventurous‚ so rich in plot‚ characters and scenery. The story is a about an ancient character named Tu Tzu-chun who supposedly lived during the Chou and Sui dynasties. Tu was in debt to a Taoist Monk that gave him wealth a couple of times over several lifetimes. Tu used his money for charity by housing and
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Patrick McCleery Essay I: Lucretius and Plato on the Mortality of the Soul In this essay it will be argued that the soul is mortal and does not survive the death of the body. As support‚ the following arguments from Lucretius will be examined: the “proof from the atomic structure of the soul‚” the “proof from parallelism of mind and body‚” the “proof from the sympatheia of mind and body‚” and the “proof from the structural connection between mind and body.” The following arguments from Plato will
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Cells are dynamic structures with constant activity The carbohydrates are unique in forming long chain polymer and also branching these chains. This is more complicated than protein where only 20 amino acids can form a long single chain. Here there can be numerous kinds of bonding forming a straight chain or long chain polymer. More over the number of repeating units are also much greater than giving the structure variation. Carbohydrates can be broken down into monosaccharides which is a single
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G-protein Coupled Receptors (GPCRs) are cell surface proteins that relay extracellular signals to the nucleus. These signals can be in the form of light‚ peptides‚ lipids‚ and sugars. Once activated by a ligand at the outer cell surface‚ GPCRs activate their cognate G-proteins that reside on the inner cell membrane. The activated G-proteins then slide along the inside of the cell membrane to activate a cascade of proteins and enzymes that in turn amplify the original message received by the GPCR
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Protein Article Research Kristen Roberts Immunity is the ability of a body to resist infection or to counter the harmful toxins produced by infecting organisms (Martin‚ Hine 2008). Antibodies and white blood cells are defensive cells and substances‚ these things produce an immune response. If such an encounter such as‚ bacteria‚ viruses‚ fungi and parasites occur can make the macrophages engulf the microorganism and secretes cytokines and chemokines. This attracts immune cells to the infected
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