Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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Red Cabbage Indicator Aim – To create a pH indicator out of a red cabbage solution and to construct a basic pH scale to determine the pH of unknown solutions. Materials - • red cabbage leaves • 250 mL beaker • hotplate or Bunsen burner‚ tripod‚ gauze mat and bench mat • 10 test-tubes – equal size • test-tube rack Methods - Part A: Making the indicator 1. Tear up one or two red cabbage leaves‚ and place them in the beaker with enough water so that the cabbage is just covered. 2. Heat the beaker
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Experimental Analysis on Enzymatic Behavior of Human and Fungal Amylase Lab name and number: Enzymes‚ Lab #5 Panther I.D: 2640403 Shayra Medal Instructor: Emily Nodine Section U21 October 26‚ 2011 X_______________________ Abstract Section The concept of this experiment was to analyze the enzyme Amylase and its environmental behavior. Amylase breaks down the biological macromolecule‚ carbohydrates‚ specifically starch into condensed subunits categorized as monosaccharaides or disaccharides
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determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient showing that as the percentage transmission will decrease with an increasing pH. This happens
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------------------------------------------------- COLORIMETRIC DETERMINATION OF pH ------------------------------------------------- Abstract This experiment examined the result of the various color indicators combined with different buffer solution‚ thereby testing the precision and accuracy of determining the pH value colorimetrically. Colorimetric method is a simple and practical procedure with an important advantage: they do not get out of order as from time to time do the pH-meters. Four buffers (Acetate buffer‚ phosphate
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Introduction When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best results when testing the pH within a solution. The pH‚ which stands for the proportion of hydrogen ions in a solution‚ could be acidic (acidosis)‚ neutral or basic (alkaline). The pH scale goes from numbers 1 through 14. A pH of 7 is neutral; meaning the amount of hydrogen
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Introduction A. Background of the study Due to the overwhelming demand of plastic bag production and its effects on our environment‚ our landfills are crammed with these non biodegradable materials. Substances used in the manufacturing brought forth various harmful chemicals which worsen over time. As the destructive compounds such as Chloroflourocarbon were introduced‚ the complete deprivation of the Ozone Layer starts. However‚ scientists and nature preservers alike managed to overthrow this
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Myrtle Freedom under threat: PH‚ 2rd deadliest country for Journalists Journalism has long been a high-risk profession‚ many journalists and reporters have put their lives on the line for a story. Journalists often have to go through extreme measures just to get information. Intensive research‚ live coverage and interviews are being demanded more and more by the public and in order to get such vital information‚ being on the scene is necessary. According to Committee to Protect Journalists (CPJ)
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University’s wooded areas‚ the oldest was created in 1989 the second was created in 1994 and the youngest plot was created in 1999. For the hypothesis to be tested‚ there were soil PH meters to test the amount of PH in each plot’s soil. The hypothesis was incorrect; there was not a significant difference in the means of the PH from the oldest plot compared to the youngest plot. Introduction The definition of vegetation is plants considered collectively especially those found in a particular area
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Process of Digestion and Absorption after eating a hamburger. After someone eats a hamburger with the works‚ they do not really think of what nutrients are in that hamburger‚ what the body does to get those nutrients out of the food‚ or even what process is used‚ they just think of how good it tasted. To get all the nutrients out of the food the human body uses processes called absorption and digestion. Some of the processes of digestion are mechanical and chemical; these are the step before the
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