Effect of enzyme concentration on rate of reaction Research question: is there a relationship between catalase concentration and the rate of reaction? Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled
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experiment the pGLO plasmid has been designed with a modified operon so that in the presence of the arabinose the bacterial cells which have been transformed by the pGLO plasmid will fluoresce due to the production of GFP. SDS-PAGE is a standard technique for determining the abundance and molecular weight of a protein using an anionic detergent (sodium dodecyl sulfate or SDS) which gives the molecules a net negative charge. The charged molecules are pipetted into a gel and then
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Introduction to Microbiology Laboratory report №10 Physical factors affecting growth microbes: Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed
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Abstract The major objective of this experiment was to observe the effects of catalase under varying controlled conditions. The scope of this experiment includes Metabolic processes‚ such as cellular respiration‚ and it poisonous byproduct hydrogen peroxide. The methodology includes procedures; multiple variables were tested in specific concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time. The major conclusion was that catalase reacts faster in warm temperatures
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Recrystallization and Melting Points Organic Chemistry 2423 Lab Report: Recrystallization Abstract: The purpose of this lab was to purify an unknown compound by recrystallization. Taking an unknown compound and identifying it by purifying it from its impurities through the use of hot gravity filtration. Then to determine the unknown you were to take the melting point. An unknown compound was recrystallized from hot water to produce 0.99 grams (67% recovery). The pure unknown compound had a melting point of 127
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The Difficulty Limited/Extensive choices pose: Its Effect on motivation and Satisfaction The Difficulty Limited and Extensive choices pose: Its Effect on motivation and Satisfaction Teagan. R. Donnelly 214282075 HPS121 Psychology B: Individual and Social Development The Difficulty Limited/Extensive choices pose: Its Effect on motivation and Satisfaction Abstract The conducted study is based on personal choice in limited and extensive choice experimental
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Purpose: To observe epithelial tissue by using a microscope to compare prepared slides. Introduction: Epithelial tissue covers the whole surface of the body. It is made up of cells closely packed and ranged in one or more layers. This tissue is specialized to form the covering or lining of all internal and external body surfaces. Epithelial tissue can be divided into two groups depending on the number of layers of which it is composes. Epithelial tissue which is only one cell thick is known as
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1. OBJECTIVES (a) To determine the equivalent circuit parameters of a 3-phase squirrel-cage induction motor from laboratory test data. (b) To determine the performance characteristics of an induction motor under load conditions. (c) To control the no-load speed of an induction motor. 2. INTRODUCTION 2.1 Three-phase squirrel-cage induction motors An induction motor is a transformer with a rotating secondary winding. It has two essential components: an outer stationary stator and an
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EXPERIMENT 1: TITLE: ISOMERISM AND KINETICS IN COORDINATION CHEMISTRY OBJECTIVE: 1. To prepare and study the kinetics of the interconversion of some of the isomers of a typical coordination compound. INTRODUCTION: Isomerism played a central role to establish the basic concept in coordination chemistry while reaction kinetics of coordination compound is known as the area of continuing intense research activity. The mechanism of the reactions of coordination compounds relevance
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Synthesis of Butyl Benzoate Using Phase Transfer Catalysis The objective of the experiment is to synthesize the butly benzoate by nucleophilic substitution and characterize it by IR spectroscopy. The percent yield of the final product is determined after the synthesis. Procedures: 2.0 mL of 1-bromobutane‚ 3.0 g of sodium benzoate‚ 5.0 mL of water‚ 4 drops of Aliquat 336‚ and a boiling stone were placed in a 50mL round-bottomed flask. The reaction mixture was refluxed for 1 hour and the flask
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