Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to
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Hand Hygiene and Aseptic Technique Keira L. Charles NURS 410: Health Deviation II Latricia Greggs‚ PhD‚ RN‚ November 17‚ 2014 Asepsis is the state of being free from living pathogenic microorganisms. Practicing hand hygiene and aseptic technique is effective in reducing the risk of associated infections in the hospital setting‚ but the adherence among health care workers are poor. Hand washing causes a significant reduction in the carriage of potential pathogens on the hands. Adhering to
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antibacterial effects--when he left a culture plate smeared with Staphylococcus bacteria on his lab bench while he went on a two-week holiday. When he returned‚ he noticed a clear halo surrounding the yellow-green growth of a mold that had accidentally contaminated the plate. Unknown to him‚ a spore of a rare variant called Penicillium notatum had drifted in from a mycology lab one floor below. Luck would have it that Fleming had decided not to store his culture in a warm incubator‚ and that London
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Lab‚ Week ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY Introduction Part I: Aseptic Technique The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique‚ which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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Coccus is a term used to describe a type of morphology that helps us distinguish different kinds of bacteria. The cell’s morphology is only one of the many ways we can differentiate the different species. Looking at these bacterium underneath the microscope is an important step to determine which kind of species it is. All cocci bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium
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Introduction The purpose of this experiment is to see how much bacteria can and will grow in common places in a typical high school. Common places can mean many things‚ including anything from water fountain spouts to computer keyboards. Such objects can hold up to 2‚700‚000 colony forming units per square inch (or CFU/in sq)(NSF). A colony forming unit is the unit used to find an estimate of the number of cells of a bacteria. This unit of measurement is commonly utilized in the subject of microbiology
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Isolation of Caffeine from Tea (12A) Introduction When a person drinks tea or coffee‚ it is not pure caffeine. It also has other natural substances. The purpose of this lab was to isolate caffeine from tea leaves. The methylene chloride removed nearly pure caffeine from the basic tea solution. Caffeine is a white‚ crystalline‚ bitter alkaloid‚ C8H10N4O2 ‚ usually derived from coffee or tea. Caffeine is used in medicine chiefly as a nervous system stimulant. Caffeine is used by humans to ward off
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methodologies and techniques performed in microbiology testing. Her close attention on my working life and my career path are very much appreciated. I would not have achieved this far and this thesis would not have been completed without all the support that I have always received from her. I would like to extend my sincere thanks to the initiator of the double international program of Master
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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