flasks containing 100ml of M1 medium (0.2g NaNO3‚ 0.05g KCl‚ 0.05g MgSO4.7H2O‚ 1g glucose‚ 0.001g FeCl3‚ 0.02 g BaCl2‚ 0.05g CaCl2) at pH 6.8 with an addition of increasing concentration of deltamethrin was prepared. 1ml of 108 fungal spore suspension cultures was added into each of these flasks. These flasks were incubated at 280± 20C on the shaker at 120rpm for 7 days. Mycelium growth was observed in each of these flasks. The mycelium growth was measured by filtering the contents using Whatman Filter
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of standard solutions whose pH is established by international agreement. A substance that is neither acidic nor basic is neutral. Solutions with a pH less than 7 are said to be acidic and solutions with a pH greater than 7 are basic or alkaline. Pure water has a pH very close to 7. The pH scale is logarithmic and as a result‚ each whole pH value below 7 is ten times more acidic than the next higher value. For example‚ pH 4 is ten times more acidic than pH 5 and 100 times (10 times 10) more acidic
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CHEMISTRY LAB REPORTS How to Write a Lab Report Lab Reports gives the explanation of the experiment done Lab reports are an essential part of all laboratory courses and usually a significant part of your grade. If your instructor gives you an outline for how to write a lab report‚ use that. Here ’s a format for a lab report you can use if you aren ’t sure what to write or need an explanation of what to include in the different parts of the report. A lab report is how you explain what you did
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Isolation‚ Expression and Cloning of Lactate Dehydrogenase Gene from Lactobacillus bulgaricus Lactobacillus bulgaricus is used for industrial production of yogurt mainly because of his fermentative capability. In this experiment‚ the gene encoding the enzyme lactate dehydrogenase [ldhA] from the bactaria was isolated and cloned. The expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E.
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Yeast Lab Report Guidelines 1. Lab reports are to be computer-generated and double-spaced. All sections of the report must be written in paragraph form. 2. Do not use encyclopedias (Internet or otherwise)‚ dictionaries ((Internet or otherwise)‚ or personal web pages as sources for the report‚ this includes Wikipedia. You may use a textbook‚ lab manual‚ and/or article(s) in a published journal. You can find journal articles by going to the library website: http://www.lib.clemson.edu/ and selecting
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a. The bacteria that causes botulism are found in soil‚ water‚ animal guts and dung. When the spores exposed to low oxygen and certain temperature‚ they produce botulinum toxin‚ able to germinate‚ metabolize‚ food sources through an aerobic respiration to produce toxin. The toxin blocks the release of acetylcholine from motor nerve endings causing paralysis symptoms. The botulism either food born‚ eating contaminated home-canned foods where the fermentation takes place. or through abrasion in the
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Introduction: Mitosis occurs in all eukaryotic cell tissues and produces genetically identical daughter cells with a complete set of chromosomes. In humans‚ mitosis produces somatic cells that are diploid‚ which means they contain two non-identical copies of each of the twenty-three chromosomes. One copy is derived from the person’s mother and the other from the person’s father. Meiosis‚ on the other hand‚ occurs only in testis and ovary tissues‚ producing sperm and ova (eggs). The gametes that
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Protocol for Lab 5 – Aerobic Respiration Part 1 Isolation of Mitochondria from Cauliflower - Weigh 50g of rosettes cut from fresh cauliflower head. - Cut rosettes and place it on ice - Prepare juice extractor by placing ice and an empty 150 ml beaker into the right compartment. - Collect pulp from left compartment and record total volume of the extract. Approx. 20ml - Filter the pulp using six layered cheese cloth and collect it in a beaker sitting on ice. - Place two 50 ml test tubes
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liters. Multiple series of solutions with different concentrations can be used by diluting the concentration. The dilution technique is: Number Moles Concentrated Solution = Number Moles Dilute Solution. An instrument called a spectrophotometer detects the amount of light that passes through the sample and the percent transmittance can be recorded from the meter. In the lab‚ multiple homogeneous solutions are made. There was not a way to determine the differences in concentrations‚ but the Spec 20
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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