Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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Lab‚ Week ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY Introduction Part I: Aseptic Technique The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique‚ which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complaining
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References: 3. Mays‚ L. W. editor. 1999. Hydraulic design handbook. McGraw-Hill Book Co 4 11. Welty‚ J.R.‚ et al‚ “Fundamental of momentum‚ heat and mass transfer”‚ 4th ed. Wiley (2001) 12
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A pasteur pipet was then used to transfer 4ml of anhydrous ether to the vial. The syringe was then utilized to transfer 0.8 ml of this solution and the reaction was allowed to proceed until the solution turns brown and cloudy. The conical vial was then rinsed with 2 ml of anhydrous ether to get any remaining solution into the
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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system only one molecule is activated for subsequent reaction. This "photo equivalence law" was derived by Albert Einstein during his development of the quantum (photon) theory of light. Absorption of visible and/or ultraviolet light by a molecule transfers all the energy of the photon to one molecule. This is sufficient energy to excite one bonding or non-bonding electron from its stable ground-state orbital arrangement to an Excited State orbital. The electrons then reorganize their bonding to stabilize
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Lab Report Microbiology Introduction Bacteria can be found almost anywhere. For human life‚ some help us‚ some hurt us‚ and others are neutral. It is now known that good bacteria‚ or normal microflora‚ can reach 1014 microbial cells. This is far more than the 1013 cells that make up the human body (Tannock‚ 1995). The total number of bacteria on Earth is estimated to be around 4-6 x 1030 (Horner-Devine‚ 2004). It is important to know the extent of bacteria‚ how they live‚ and how they are
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