In this lab we employed various assays utilizing a biuret reagent‚ coomassie brilliant blue reagent‚ and ultraviolet light in order to determine the identity of six unknown solutions and the concentration of a bovine serum albumin sample. We were given three samples that lacked protein‚ and three samples containing proteins‚ and using a spectrophotometer we assessed the amount of light absorbed versus the light transmitted‚ based on the principles of the Beer-Lambert Law. The three proteins used
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A small amount of the unknown (approximately the tip of the spatula was used for all the pigments) was added into each clean dry test tube. For the first test 1 mL of 3 M of HNO3 was added. As soon as the acid came into with the solid‚ the pigment was observed to dissolve while also turning the solution into a pale clear colour. The same amount was used for the HCl reagent. As soon as the acid touched the pigment it yielded in a clear solution (solid dissolved after a few shakes). When the KI was
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ANALYSIS OF ASPIRIN: SPECTROPHOTOMETRY The purpose of this experiment is to evaluate the percent aspirin on a commercial aspirin tablet using an instrumental method‚ spectrophotometry. In a spectrophotometer‚ light from a strong lamp passes through a monochromator‚ which breaks the light into its component colors using a grating‚ then uses mirrors and slits to direct a light beam of the desired wavelength through the sample compartment‚ where you place a tube (cuvette) of solution. A detector
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OF ASPIRIN Aspirin in general is a safe drug but the ability to prevent blood clots is connected to some side effects. Aspirin can cause firstly stomach irritation. That’s means aspirin can irritate your stomach which has as a result pain‚ nausea‚ vomiting or hearburn. Sometimes can have other symptoms like bleeding‚ holes in stomach or intestines. In order to minimize these problems it should be better to have a full stomach. Also another problem that comes from aspirin can
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Multistep Synthesis of Benzilic Acid The synthesis of several complex organic compounds follows a multistep synthesis. "Multistep synthesis" refers to the procedure in which the product of one reaction serves as the starting material in the subsequent reaction. The multistep synthesis of benzilic acid begins with a conversion benzaldehyde to benzoin through a condensation reaction. The benzoin then oxidizes into benzil‚ which undergoes rearrangement to give benzilic acid. Benzoin Synthesis * When
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Purpose Acetophenetidin can be formed through two methods‚ Williamson ether synthesis and amide synthesis. By working in groups of two we were able to complete both methods of synthesis routes. The end result should be the synthesis of a similar product‚ by verification between the two individuals. Reaction Experiment and Observations Amide Synthesis of Acetophenetidin The Synthesis reaction began by removing the colored impurities from the p-phenetidine‚ accomplished by mixing
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Lab #28 Conservation of Mass Ashleigh Bublinec Serena Contreras
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mL of unknown #12. The flask was then covered and had a hole poked through the foil. Next‚ it was placed into boiling water. After the liquid was fully evaporated it was taken out‚ wiped dry and‚ allowed to cool. Then the mass was measured. Then the lab station was cleaned and the waste properly disposed of.
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is associated with lactase persistence is the located upstream of the LCT gene. In this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele
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The Grignard Synthesis of Triphenylmethanol Abstract: Grignard synthesis of triphenylmethanol was achieved by use of the Grignard reagent phenyl magesium brominde. The organometallic grignard reagent was synthesized by use of a reflux apparatus recrystallization techniques. Once synthesized it was used in a Grignard reaction that involved nucleophilic addition to a carbonyl in order to make triphenylmethanol. The final product was solidified and recrystallized and spectral data was
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