Apparatus: -Osborne Reynolds apparatus. -Hydraulics Bench. -measuring cylinder. -Stopwatch. -Vegetable Dye. -Thermometer. 4. Procedure: * Fill the reservoir with dye‚ position the apparatus on the bench and connect the inlet pipe to the bench feed. Lower the dye injector until it is just above the bell moth inlet. Close the control valve. Open bench inlet valve and slowly fill the head tank to the overflow level
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separate different-sized molecules in a porous‚ sponge-like matrix. 2. What is the purpose of the agarose gel? It is used to separate DNA molecules that range in different lengths. 3. What is the purpose of adding blue “tracking” dye to the DNA samples? The blue tracking dye is added to help load the samples easily and helps able to see the DNA moving through the gel. 4. Explain why DNA has an overall negative charge. The phosphate groups in the DNA backbone carry negatively-charged oxygens‚ which
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have to take yellow and blue to make that green. Plus the pigments that were shown on the paper. (5 points) 6. You are given an unknown type of clothing dye. How could you use the procedures in this lab to see if this dye is a mixture? Answer: In order to find out if the clothing dye is a mixture‚ you would rub a small amount of the dye on the filter paper just like you would the dot of ink. Then place it in your beaker or some container of
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membrane structure Introduction Beetroot Pigments Beetroots contain Betalains which are the red pigments present in the cell vacuole. Betalains are soluble in water and they contain nitrogen. Betalains extracted from beetroot is commonly used as food dye because it is not known to cause any allergic reactions. Beetroot Picture taken from http://tipdeck/how-to-cook-beet-root Structure of Betalain Picture taken from http://en.wikipedia.org/wiki/File:Betanin.png Cell Membrane Cell membrane
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define Beer’s law and define the relationship between absorbance and transmittance. Other learning objectives are to create a Beer’s law plot for a series of samples with known concentrations‚ collect spectrophotomic data from unknown and known FDC blue dye samples‚ perform serial dilutions‚ calculate concentrations‚ perform linear regression and determine the equation of a best fit line. DISCUSSION/OBSERVATION A solution is composed of a solute dissolved into a solvent. The most common solvent is water
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Investigating the effect of pH on the activity of the enzyme catalase. Introduction Hydrogen peroxide (H2O2) is a very pale blue liquid which appears colourless in a dilute solution‚ slightly more viscous than water. It is a weak acid. It has strong oxidizing properties and is therefore a powerful bleaching agent that is mostly used for bleaching paper. Catalase is a common enzyme found in all living organisms. Its functions include the conversion of Hydrogen Peroxide‚ a powerful and potentially
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lamellae which are layers of mineralised matrix. These lamellae are pink in colour which shows the eosin dye of H&E is staining this structure. This means that the lamellae of the osteons are acidophilic structures. The last structure of the osteon that can be seen in this figure is the cement line. This is a thin line that surrounds the entire osteon. It is purple in colour which shows the haemotoxylin dye of H&E is staining the cement line. This is turn means that this part of the osteon is a basophilic
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of phyto diversity along with some highly valuable endemic and endangered species. The forest is deciduous type but mixed with some ever green elements. It is a source of non-wood forest products (NWFP) like fiber‚ fuel‚ wood‚ gum‚ resin‚ vegetable‚ dyes‚ oil‚ honey‚ medicinal plants‚ bamboo‚ etc. Key words: Ethnobotany; Andhra Pradesh; Kambakam Hills. INTRODUCTION: Ethnic people are confined to definite geographical areas and speak common dialect‚ are culturally homogenous and evince a unifying
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Isolation and purification of bovine milk α-lactalbumin. Abstract: The Bradford assay‚ a colorimetric protein assay‚ is based on an absorbance shift of the dye Coomassie Brilliant Blue G-250 in which under acidic conditions the red form of the dye is converted into its bluer form to bind to the protein being assayed. The (bound) form of the dye has an absorption spectrum maximum historically held to be at 595 nm. Objective: To determine the technique to a series of protein purification steps
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Why does colour leak out of cooked beetroot? Introduction When cooking a beetroot you are recommended not to remove the outer skin and not to cut off the entire stalk if you do not want the red dye in the cooking water. Beetroot contains a red pigment called betalain‚ which is in the cell vacuole. Normally the betalain stays inside the cell‚ however when the cell is exposed to extreme temperatures they leak out. The reason why they leak out is because of the cell membrane structure. When the
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