Biochemical Test – Enterobacteriaceae Bacterial spp incapable of fermenting glucose cannot utilize lactose. 2 enzymes necessary for a bacterium to take up lactose: A. β-galactoside permease – facilitates the entry of lactose molecules to bac cell wall B. β-galactosidase – breaks down lactose into β-D glucose and β-D galacatose LF possess both enzymes NLF do not possess β-galactosidase LLF do not possess β-galactoside permease Glucose fermenters only (true enteric pathogen)
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References: Bergmans‚ H. E.‚ Van Die‚ I. M.‚ & Hoekstra‚ W. P. (1981). Transformation in Escherichia coli: Stages in the Process Panja‚ S.‚ Aich‚ P.‚ Jana‚ B.‚ & Basu‚ T. (2008). How does plasmid DNA penetrate cell membranes in artificial transformation process of Escherichia coli? Molecular Singh‚ M.‚ Yadav‚ A.‚ Ma‚ X.‚ & Amoah‚ E. (2010). Plasmid DNA Transformation in Escherichia coli: Effect of Heat Shock Temperature‚ Duration‚ and Cold Incubation Wilfinger‚ W. W.‚ Mackey‚ K
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This article was downloaded by: [Universiti Teknologi Malaysia] On: 03 January 2015‚ At: 02:59 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House‚ 37-41 Mortimer Street‚ London W1T 3JH‚ UK Bioengineered Publication details‚ including instructions for authors and subscription information: http://www.tandfonline.com/loi/kbie20 Cloning‚ soluble expression‚ and purification of the RNA polymerase II subunit RPB5 from Saccharomyces
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1 Detecting glowing E.Coli Colonies by making recombinant DNA from the lux operon of Vibrio Fischeri to pGEM. Liao‚ Tffany The marine bacterium Vibrio Fischeri produced bioluminescence effect due to lux operon transcription. The purpose of the experiment is to create a genomic library of Vibrio DNA and clone the lux operon by making Recombinant DNA and transform into another organism‚ E. Coli. Chromosomal DNA of vibrio fischeri was first extracted and digested with restriction enzyme Sal I‚ then
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ANTI-INFECTIVES Anti-infective agents are drugs that are designed to act selectively on foreign organisms that have invaded and infected the body of a human host. Ideally‚ these drugs would be toxic to the infecting organisms only and would have no effect on the host (or human) cells. OVERVIEW • Antimicrobial therapy (often termed “antibiotic therapy”) is the use of medications to treat infections caused by bacteria‚ viruses‚ and fungi. • Antimicrobial must use selective toxicity to kill
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MASINDE MULIRO UNIVERSITY OF SCIENCE AND TECHNOLOGY P.O. BOX 190-50100 KAKAMEGA FACULTY OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES COURSE TILTE: INDUSTRIAL/FIELD ATTACHMENT COURSE CODE: SBL326 NAME: PAMELA K. MUKWEYI REG. No.: BTE/0517/08 DURATION: 9TH MAY – 20TH JULY 2012 SUBMISSION DATE: ATTACHMENT PLACE:CENTRE FOR MICROBIOLOGY RESEARCH- KEMRI Scope/purpose The Industrial Attachment program fulfils part of the requirement in pursuing the degree
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Castillo Tania Castillo Epidemiology Report of Tuberculosis Tuberculosis (TB) is a chronic bacterial disease that is highly in development countries. This disease is caused by a bacterium called Mycobacterium tuberculosis‚ an acid-fast aerobic bacillus‚ which is prevalent in tropical areas across the globe. For instance‚ the majority of the cases occur in malnourish individuals‚ immigrants and elderly persons. Most of the person’s who has the disease are concurrent for many years‚ and the reactivation
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Public Health at Washington University in St. Louis. While Rife was acquiring the technical skills and instruments he needed to isolate the cancer virus‚ Dr. Kendall was developing a protein culture medium for cultivation of the typhoid Bacillus. The typhoid Bacillus was usually large enough to be viewed through ordinary research microscopes of the time. However‚ when he used his Medium K‚ this bacterium changed into a form that was not visible or “non-filterable”. Then‚ it would morph back into a
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To scrutinize the color potency depth of the azo dye Remazol Black-B decolorizing activity by an effectual bacterial isolates‚ liquid medium was used. Based on the relative aptitude of the decolorization of diverse isolates‚ three most excellent performing bacterial isolates (1‚ 2 & 3) with more than 90% of decolorizing activity were preferred for more experiments (Data not shown). 3.1 Biodecolorization of Remazol Black-B by selected bacterial isolates Microbial decolorization of Remazol Black-B
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are bacterial SHAPES? (Select all that are correct. Be sure to select the appropriate scientific name. You lose points for choosing incorrectly or for not choosing a correct choice.)A) Vibrio B) Strepto C) Spirillum D) Staphylo E) Cocci F) Bacillus G) Diplo3.A chromophore is the:A) transparent component of the dye B) the part of the dye that does not bind. C) the colored portion to the dye. D) part of the dye that is not seen. E) the part of the dye that is not used up.4.Which type of
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