Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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membrane-active compounds can enhance the activity of conventional antibiotics‚ eliminate natural resistance to specific antibiotics (reversal of resistance) and exhibit strong activity against multidrug-resistant forms of Staphylococcus aureus‚ Escherichia coli‚ Salmonella spp.‚ Mycobacterium tuberculosis‚
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1) Microscope 2) Lab Coat 3) Gram Staining Lab 4) 2 Slides 5) Gram Stain Solution 6) Gram crystal violet 7) Gram iodine 8) Burner 9) Alcohol 10) Water 11) Staphylococcus epidermidis 12) Escherichia coli 13) Bacillus subtillis 14) Gram Safrain 15) Procedures First I get all my materials to get started. I make sure I turn on the burner and sterilize all my materials. I then smear E.C‚ BS‚ & SA and a mixture of all on 2 slides separated
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Introduction: Chemical methods of control: Antimicrobial drugs‚ involves the use of chemicals to prevent and treat infectious diseases. Pasteur and others observed that infecting an animal with Pseudomonas aeruginosa protected the animal against Bacillus anthracis. Later‚ the word “antibiosis” (against life) for this inhibition and called the inhibiting substance “Antibiotic”. As researchers found out more and more about these chemicals they were able to discover that antibiotics are chemicals or
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Abstract: The objective of the experiment is to learn how to use a microscope while viewing various types of bacteria cultures. Prepared slides are used to view bacterial cultures under a microscope that would normally be invisible to the naked eye. Bacterial shapes and arrangements or morphology are observed in prepared slides and fresh yogurt culture. Purpose: The experiment is meant to determine the shapes of bacteria under different magnifications when using a microscope; 10X‚ 40X‚ and 100X
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Exercise 8-B Differential Staining Gram Staining and Acid Fast Staining Introduction: Differential Staining‚ one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans
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Department of Food Science and Technology College of Agriculture and Food Science Visayas State University Baybay City‚ Leyte Microbiology of Fish and Fish Products Introduction: Fish is a major staple food in most parts of the world and are second only to meat as the major animal protein in most diets. Whilst foods such as ‘meat’ form relatively well -defined groups of raw materials‚ ‘fish’ constitutes a large range of types (some sources recognise over 20‚000 identified species)‚ caught over
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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synthesized silver nanoparticles from aqueous extract of Gum of fresh Coconut was tested by the disc diffusion method against an antibiotic‚ ampicillin(1µg/µl) as a standard drug. The antibiotic resistant bacteria viz.‚ Staphylococcus aureus‚ Bacillus subtilus‚ Escherichia coli and Klebsiella pneumonia were used for the measurement of antibacterial activity‚ and the plates were incubated at 370C overnight to know the zone of inhibition. 2.2. Cell Culture and Nanoparticles Treatment For cell culture‚ selective
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microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside. 2. Place 1 to
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