MICROBIAL SURVEY‚ SMEAR PREPARATION‚ AND SIMPLE STAIN Instructional Objectives 1. Define Roccal = green‚ liquid disinfectant. Pathogen = an agent which causes disease. Wet Mount Slide = a microscope slide of a liquid specimen covered with a cover glass. Yeast = a single celled fungi. Budding = a true characteristic method of asexual reproduction among yeasts where budding of a new cell from a parent cell can be observed. Mold = multicellular masses of filamentous fungal growth. Hyphae
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|Medium |Generation Time (minutes) | |Escherichia coli |Glucose-salts |17 | |Bacillus megaterium |Sucrose-salts |25 | |Streptococcus lactis |Milk |26
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Experiment 9: Growth curve of Serratia marcescens Abstract Bacteria grows by binary fission. The aim of this experiment is to follow the growth of Serratia marcescens in nutrient broth at 37oCby recording the changes in turbidity (cloudiness) by measuring the absorbance of visible light (600 nm) and also to prove that there is an increase in the cell number and not just in mass during the growth. In the experiment we measure the full growth curve of Serratia marcescens by measuring the absorbance
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turn the agar plate over and divide the plate into four quadrants and label the agar plate whether you used the E. coli or B. megaterium and number the quadrants 1 through 4. Please keep in mind that one pair will test the E. coli and Environment 1 or 2‚ and one pair will test B. megaterium and Environment 1 or 2. After‚ you will need to swab the E. coli and B. megaterium on two different nutrient agar plates using a sterile disposable inoculating loop. Remember not to dig in into the agar or the
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This was compleated by growing the bacteria on a petri dish with agar‚ gram staining‚ then examning under a microscope. The bacteria identified was M. Roseus and B. Megaterium. The identity of these bacteria was mostly determined by the positve gram stain results and the shape they where under the microscope. Both of these bacteria where identified as gram positive (as shown in the table previously). This was determined
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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Alternative medicine has been around for centuries‚ although it has just started to become very popular in countries such as The United States. Many people now are following the trend without knowing anything about alternative medicine. People should be aware of the benefits as well as the precautions involved in taking these natural remedies. The most common form of alternative medicine nowadays is herbal medicines. These natural remedies can be found in millions of American homes today. Herbal
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Available online at www.sciencedirect.com Polyhydroxyalkanoates: bioplastics with a green agenda Tajalli Keshavarz and Ipsita Roy Production of polyhydroxyalkanoates (PHAs) has been investigated for more than eighty years but recently a number of factors including increase in the price of crude oil and public awareness of the environmental issues have become a notable driving force for extended research on biopolymers. The versatility of PHAs has made them good candidates for the study
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granules in the microscope as highly refractive inclusions. However‚ the first example of PHA came in existence until almost four decades later in 1926 when a French microbiologist Maurice Lemoigne discovered that a gram negative bacterium Bacillus megaterium accumulated intracellular granules of a polyester called polyhydroxy-3-butyrate (PHB) (Amara et al.‚ 2011). PHA accumulation ability has been reported for many microorganisms including Gram-negative and Gram-positive species (i.e. autotrophic
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Names: ______________________________ ______________________________ ______________________________ ______________________________ Week 1 Lab Goals: 1. Choose your lab group. 2. Learn about lab safety. **IMPORTANT – All students are expected to wear a lab coat and goggles in lab at ALL times. As well‚ you are not permitted to wear shorts or open-toed shoes. If you are not dressed appropriately for lab‚ your TA will dismiss you. You are permitted to return to lab with proper attire
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