UNIVERSITY OF DAR –ES-SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCES DEPARTMENT OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY MC206: FOOD MICROBIOLOGY PRACTICALS PRACTICAL 1 MICROORGANISMS IN THE ENVIRONMENT GROUP #:1 NAME: DUSENGEMUNGU Léonce REG #: 2011-04-07086 COURSE INSTRUCTOR: Dr Mugassa S.T Rubindamayugi
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types of bacteria. Procedure: 1. Focus the microscope on a slide containing the letter ‘e’. 2. Focus the microscope on 6 other prepared slides. 3. Focus the microscope on slides Bacteria spirillum‚ Bacteria coccus‚ and Bacteria bacillus. 4. Prepare a fresh yoghurt slide for viewing under the microscope‚ and then compare what you see to what you see on your prepared slide from the lab kit. Data/Observations 1. The microscope was focused on the “e” slide and the slide
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coli). Bacillus subtilis is a gram-positive rod-shaped bacterium‚ typically found in vegetation and soil. (Entrez Genome Project). E. coli is gram-negative bacterium commonly found in the gut of warm-blooded organisms. (National Health Service) HYPOTHESIS If we
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Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters
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has more than 100 phytochemicals. It is used as sugar substitute. The extracts from leaves are used for the microbial assay and study the antimicrobial activity. The microbes used for the testing the activities are E.coli‚ Staphylococcus aureus‚ Bacillus subtilis‚ Salmonella typhi‚ Aspergillusniger‚ Candida parapsilosis‚ Trichophyton rubrum. With different concentrations of the extracts starting from 2.5µg/ml‚ 5.0µg/ml‚ 7.5µg/ml‚ 1.0µg/ml and the highest activity is shown by Staphylococcus aureus
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a sample of contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that the bacterium is of the bacillus family due to the rod shape. Due to multiple complications with the PCR‚ a new hypothesis couldn’t be formulated. Methods: In this experiment‚ a 1 gram sample of contaminated dirt was taken and mixed with 100 mL of sterile water then shaken. After
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Canada Spees Lab 2 Prokaryotes Purpose: Performing this lab will help me better understand the characteristics of prokaryotes and compare them with eukaryotes. This lab will also help me visualize and better understand the physical description‚ and the different types of bacteria and how they stain‚ whether it be gram positive or gram negative. Hypothesis: If I read the textbook and use the resource websites‚ then I will be able to better understand prokaryotes and describe the structural characteristics
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conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify the basic shape and size of the microbe. In this experiment‚ the microbes E. coli‚ Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. These staining techniques are the correct way to identify the shape and size of microbes.
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Amanda Lab Report 1 Microbiology Laboratory Title – Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories
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Typhoid Fever Typhoid fever is a life-threatening disease of the intestinal system caused by the typhoid bacillus‚ Salmonella typhosa‚ which lives only in humans who carry it in their bloodstream and intestinal tract. Typhoid fever is spread when the bacteria is "shed" by infected people who handle food or fluids without washing their hands‚ or when sewage carrying the bacteria contaminates water‚ milk‚ and other foods. Although relatively rare since the advent of vaccines and improvement
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