Aseptic technique Michele Pearson‚ Leah Christine Silver‚ and William Jarvis Purpose Aseptic technique is employed to maximize and maintain asepsis‚ the absence of pathogenic organisms‚ in the clinical setting. The goals of aseptic technique are to protect the patient from infection and to prevent the spread of pathogens. Often‚ practices that clean (remove dirt and other impurities)‚ sanitize (reduce the number of microorganisms to safe levels)‚ or disinfect (remove most microorganisms but not
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desired specimen. It also took practice in adjusting the light source so as to have the proper contrast and brightness. In the end the practice using the prepared slides allowed for clear observation of the various bacterial cell shapes such as cocci‚ bacillus‚ and spirillium. These shapes were more easily identified in the prepared slides and this knowledge was used to view similar structures in the fresh yogurt slide. Finally‚ analysis of a blood smear was interesting because it was initially very hard
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Microbiology UNKNOWN LAB REPORT Kateryna Petrakova INTRODUCTION Microorganisms cause a great amount of diseases. For healthcare providers it is very important to be aware of what organisms are pathogenic and cause a disease and‚ therefore‚ to find an appropriate treatment. Different microorganisms require various environments in order to replicate and to become dangerous for a person’s health. An integral part of any medical treatment is to be able to recognize and
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Saccharification of starch for alcohol production 4. Brewing What organisms are responsible for amylase production? Although many microorganisms produce this enzyme‚ the ones most commonly used for their industrial production are Bacillus subtilis‚ Bacillus licheniformis‚ Bacillus amyloliquifaciens and Aspergillus niger General Lab Requirements: • Autoclave or pressure cooker • Hot Plate or Microwave oven • Nutrient Agar powder • Potato Dextrose Agar powder • Soluble starch • Weighing scales •
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121-125. 8. Serrano M et al‚ (1999). The Microbial World. Available on <http://www.microbiologytext.com/index.php?module=Book&func=displayarticle&art_id=69> [Accessed on 7 July 2012]. 9. John L (2001). Bacteriology 102: Isolation of Bacillus. Available on <http://www.splammo.net/bact102/102bacillus.html> [Accessed on 7 July 2012]
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into food and water also absorbed or injected in the skin. Some common biological agents that are heard in the news and also used in warfare include Anthrax‚ Cholera‚ Clostridium Perfringeus Toxin‚ Meliodosis idosis‚ and Staphylococcal. Anthrax (Bacillus anthracis) develops when a bacterial organism from infected animal tissues becomes deposited under
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experiment carbohydrate catabolism was first tested under aerobic conditions. We began this by drawing three sections on the bottom of the petri plate‚ dividing it into three sections. Using a sterile loop‚ we streaked a single line of each culture Bacillus subtilis‚ Pseudomonal aeruginosa‚ and Escherichia coli‚ to each of the separate sections. The plate was incubated until the next lab period‚ where we then flooded the plate with Iodine and were able to observe and record starch Hydrolysis. (Table
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Microscope 2) Lab Coat 3) Gram Staining Lab 4) 2 Slides 5) Gram Stain Solution 6) Gram crystal violet 7) Gram iodine 8) Burner 9) Alcohol 10) Water 11) Staphylococcus epidermidis 12) Escherichia coli 13) Bacillus subtillis 14) Gram Safrain 15) Procedures First I get all my materials to get started. I make sure I turn on the burner and sterilize all my materials. I then smear E.C‚ BS‚ & SA and a mixture of all on 2 slides separated. Then I air-dry
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2: To prepare concrete using the cement and solution (bacterial) in optimum ratio and checking its effectiveness from strength and durability perspective. Till now Stage 1 is partially over: We have made use of this bacterial solution(Emulsion: Bacillus culture+ nutrient broth+ sucrose+ urea+ calcium chloride+ antifungal agent(griseofulvin)to heal the existing cracks in the concrete walls. For achieving high 7 days we have made use of bacterial solution in some fixed proportion with water. The
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Department of Food Science and Technology College of Agriculture and Food Science Visayas State University Baybay City‚ Leyte Microbiology of Fish and Fish Products Introduction: Fish is a major staple food in most parts of the world and are second only to meat as the major animal protein in most diets. Whilst foods such as ‘meat’ form relatively well -defined groups of raw materials‚ ‘fish’ constitutes a large range of types (some sources recognise over 20‚000 identified species)‚ caught over
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