culture from the agar cultures 3. It then mix with the drop of water and then proceeds as broth cultures. SIMPLE STAINING TECHNIQUES Material: 1. 24 hours broth cultures of a. E. coli b. Staph. aureus 2. 24 hours nutrient agar slants of a. Bacillus subtilis b. Pseudomonas aeruginosa 3. Slides 4. Inoculating loop 5. Dye solution a. Crystal violet b. Methylene blue c. Carbol fuchsin 6. Test tubes Procedure: 1. By using inoculating loop a minute of bacterial was transferred onto the
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biochemical reactions. Qualitative observations were made on the tests in identification process. The data from experimentation provided sufficient results to suggest that the identity of unknown bacterium was the gram-positive spore forming bacteria‚ Bacillus Subtilis. Introduction. One of the main responsibilities of a medical lab is to determine the identity of pathogenic bacteria. It is important to determine the specific type of bacterium causing disease so the physician is able to correctly
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Research in Biotechnology‚ 3(6): 51-58‚ 2012 ISSN: 2229-791X www.researchinbiotechnology.com Regular Article Isolation and identification of a new Bacillus strain for amylase production Yasser Bakri*1‚ Hassan Ammouneh1‚ Samir El-Khouri1‚ Muhanad Harba1 and Philippe Thonart2 1Department of Molecular Biology and Biotechnology‚ AECS‚ Damascus‚ Syria‚ P.O.Box 6091 Wallon de Biologie Industrielle‚ Unité de Bio-industrie‚ Faculté de AgroBiotechnologie de Gembloux‚ Université de Liège
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themselves to substrate molecules and lowering the activation energy of a reaction (Alberts et.al‚ 2010 p.90). The purpose of the lab was to test how temperature will affect the rate at which starch hydrolyzed. In the experiment conducted‚ bacterium; Bacillus Licheniformis and the fungus; Aspergillus Oryzae was used to examine how changes in temperature of the enzymes affects the rate of the reaction with starch. Amylase is an enzyme‚ found chiefly in saliva and pancreatic fluid‚ that converts starch
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Dobrinka Atanasova Anthrax Anthrax is acute infectious disease that affects humans and other mammals. The causative agent of Anthrax is Bacillus anthracis. Bacillus anthrasic is a rod-shaped bacterium that forms spores (3). As we know‚ spores can survive many‚ many years in harsh conditions. Furthermore‚ the bacterium is also Gram-positive‚ encapsulated and aerobic (2). Anthrax can enter and affect the body via the skin‚ the lungs or gastrointestinal tract. The first one‚ the skin‚ is the least
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It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as the hay bacillus or the grass bacillus. It is gram and catalase positive. It is rod-shaped‚ with the ability to form a tough‚ protective‚ shield around itself. It can tolerate extreme environmental conditions‚ and is an obligate aerob. The antibiotic
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Preston Pictures are at bottom Anthrax is a infectious disease caused by a bacterium called Bacillus anthracis. Anthrax most commonly occurs in livestock‚ but it can also occur in humans when they are exposed to infected animals or to tissue from infected animals or when anthrax spores are used as a bioterrorist weapon.Anthrax is most common in agricultural regions where it occurs in animals. Although anthrax can be found globally‚ it is more often a risk in countries with less s98\standardized
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Bacillus‚ Mycoplasma‚ and Escherichia In a clinical setting signs and symptoms can lead a care taker down a treatment path‚ but if a bacterial infection is suspected‚ a definitive answer from the “lab” is needed before prescribing medications. In this instance a yellow sputum was produced by the patient‚ which will be taken to the lab an analyzed under a microscope. There are three suspect bacteria‚ which are Bacillus‚ Mycoplasma‚ and Escherichia. A discussion of staining procedures‚ as well as
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INTRODUCTION Cassia fistula Linn. (Leguminosae) is a very common plant and is widely known for its medicinal properties. In the Indian literature‚ this plant has been described to be useful against skin diseases‚ liver troubles‚ tuberculous glands and its use in the treatment of rheumatism‚ hematemesis‚ pruritus‚ leucoderma‚ and diabetes.[1‚2] Besides‚ it has been found to exhibit anti-inflammatory and hypoglycemic activity and widely used as a mild laxative suitable for children and pregnant women
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where it grows‚ it’s incubation period‚ the signs after the incubation period‚ and how to possibly control it‚ along with the difficulties of controlling this disease. Anthrax is a zoonotic disease caused by exotoxins released by the bacteria‚ Bacillus anthracis‚ a spore-forming bacterium (Islam‚ 2013). B. anthracis is a Gram-positive‚ rod-shaped‚ spore-forming bacterium. It is a causative agent of anthrax‚ but rapidly an infectious disease that affects both animals and humans (Liu‚ 2014 & Elad
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