Study Guide to Help with Laboratory Exam 1 Microbiology 1214 A partial study guide only. Not intended to replace the review sheets or the “Know the following” sections on your labs. These slides are not presented in any particular order. Name this Procedure Identify the two types of bacteria present by shape and gram stain. In a gram stain what is the primary stain? The mordant? The counterstain? How does this differ from a simple stain? Identify the shape and gram stain of the bacteria present
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Objectives 1. To determine biochemical activities of microorganisms. 2. To observe the product of biochemical activities of microorganisms. 3. To learn the skills of inoculation agar tubes and agar plates. Introduction Microorganisms are able to carry out different biochemical activities with the ease of different enzymes. Each of these enzymes carries out one specific type of the chemical transformation. They convert substrates into product. A) Carbohydrates Fermentation Microorganisms utilize
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green synthesized silver nanoparticles from aqueous extract of Gum of fresh Coconut was tested by the disc diffusion method against an antibiotic‚ ampicillin(1µg/µl) as a standard drug. The antibiotic resistant bacteria viz.‚ Staphylococcus aureus‚ Bacillus subtilus‚ Escherichia coli and Klebsiella pneumonia were used for the measurement of antibacterial activity‚ and the plates were incubated at 370C overnight to know the zone of inhibition. 2.2. Cell Culture and Nanoparticles Treatment For cell
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Lab #1 (16th October‚ 2012) Safety in the Microbiology Lab * Clinical specimens received from patients pose a hazard to personnel health because of the infectious agents they may contain. * Spurred by the spread of HIV/AIDS‚ the CDC has recommended safety precautions concerning the handling of patient materials by health care workers. * Universal precaution (or blood and body fluid precaution). * Emphasis on good knowledge and common sense. * Safety is everybody’s business
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Emergency Preparedness and Response Protecting the public’s health historically has been a state and local responsibility. However‚ the growing threat of bioterrorism has highlighted the importance of a strong public health infrastructure to the nation’s homeland security and has focused increased attention on the preparedness of the public health system (Frist‚ 2011). Since the 2001 anthrax attacks Congress has passed new legislation to increase the strength of the nation’s public health system
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developing drought and herbicide resistant crops. Through genetic engineering‚ scientists have been able to introduce traits into existing genes to make crops resistant to drought and herbicides. One good example is the use of Bacillus Thuringiensis‚ commonly known as Bt. Bacillus Thuringiensis‚ is a bacterium that dwells in soil. It acts as a reservoir “of cry toxins and cry genes for production of biological insecticides and insect-resistant genetically modified crops.” How has gene technology benefited
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Whooping Cough A. Name the bacteria that cause this childhood disease. What is its morphology? bacillus bordetella pertussis. B. pertussis is an extremely small‚ strictly aerobic‚ Gram negative‚ cocobacillus short rod. What does “Wooping Cough” mean? Pertussis is a highly contagious bacterial disease that causes uncontrollable‚ violent coughing. The coughing can make it hard to breathe. A deep "whooping" sound is often heard when the patient tries to take a breath. Describe in detail the
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the medium immediately surrounding the colonies after addition of iodine on PDA plate. Organism Result + or - Appearance/Observations Bacillus cereus Escherichia coli B. Gelatin Hydrolysis (gelatinase production) Note: whether the gelatin deep remains solid or has been liquefied Organism Result + or - Appearance/Observations Bacillus subtilis Escherichia coli C. Coagualse: identification of Staphylococcus aureus Note: whether the rabbit plasma has
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Exopolymeric substances are required for Calcium carbonate precipitation Abstract: Introduction: Different minerals precipitation by microbes is a common phenomenon‚ and carbonates are most common mineral formed. Many micro-organisms are having the ability in undergoing the process of mineralization‚ although different minerals have been precipitated by microbes which include carbonates‚ sulphates‚ silicates etc [1]. Amongst all these‚ carbonates are the most common minerals formed. Carbonate precipitation
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Diagnosis of latent TB relies on the tuberculin skin test (TST) and/or blood tests. Antibiotic resistance is a growing problem in multiple drug-resistant tuberculosis (MDR-TB) infections. Prevention relies on screening programs and vaccination with the bacillus Calmette–Guérin vaccine. One third of the world’s population is thought to have been infected with M. tuberculosis‚ with new infections occurring in about 1% of the population each year.[4] More people in the developing world contract tuberculosis
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