energy either sunlight or chemicals‚ nutritional requisites and biochemical actions. We have a patient that may be infected with an unknown bacterial infection; we’ve collected a sputum sample and will be testing for one of the following genera: Bacillus‚ Escherichia and Mycoplasma. In order to determine which one of the three may be the source of the infection we have to recognize the characteristics of each one and the staining protocol needed to identify them. All three of the above mentioned
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diaphragm aperture; the difference in lighting between adjacent areas of the specimen C. Describe your observations from fresh yogurt slide you prepared in Part III. While viewing under 4x magnification‚ you can see the presence of cocci and bacillus bacteria strains. After increasing the magnification to 10x‚ you can view these shapes more clearly‚ but it is not until 40x magnification that you can see the cooci exist in pairs and strands‚ in other words‚ diplococci and streptococci are present
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would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly with my results but Lysol was only 4mm more sustainable then Windex. Lysol with the biggest zone of inhibition of 28mm allowing this disinfectant to kill the most Bacillus subtilis bacteria. Still‚ Lysol was succeeded to work
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previous study (Jariyal et al.‚ 2014)‚ comparative analysis identified bacterial species Brevibacterium frigoritolerans‚ Bacillus aerophilus and Pseudomonas fulva as most potent phorate degrader‚ caused complete metabolization of phorate in 13 day. In view of the above‚ the phorate degradation ability of these bacterial strain could be arranged as Pseudomonas fulva strain Imbl 5.1> Bacillus aerophilus strain Imbl 4.1> Brevibacterium frigoritolerans strain Imbl 2.1. In view of the above‚ further studies
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accounted by the slow movement of molecules that decreases enzyme and substrate collision. The difference in optimal temperature between the fungal and bacterial amylase may be due to the different environments that each enzyme is naturally found in. Bacillus licheniformis is found in a wider range of environments so its enzymes have to survive a higher range of temperatures. Where as Aspergillus oryzae is found in lower temperature habitats preventing its enzymes from tolerating a wider range of temperatures
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Several cells majority Bacillus and cocci cells. D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so‚ describe them. Only perhaps that there was far more Cocci cells then Bacillus E. Describe the four main bacterial shapes 1. Cocci - Spherical or oval bacteria having one of several distinct arrangements based on their planes of division 2. Bacillus - Rod shaped bacteria. Bacilli all divide in one plane producing a bacillus‚ streptobacillus
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Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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a good bioterrorism agent. How is Anthrax Caused? Anthrax is caused by the bacterium[2] ‘Bacillus anthracis’ that is highly lethal in its virulent form. Anthrax most commonly occurs in wild and domestic animals‚ but it can also occur in humans when they are exposed to infected animals‚ tissue from infected animals‚ or high concentrations of anthrax spores.[3] Bacillus Anthracis Bacillus anthracis‚ the bacterium which causes anthrax‚ is rod-shaped and about 1 by 6 micrometres in size
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microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside. 2. Place 1 to
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Observing Bacteria Kelli Jo Simco Microbiology Due: 2/8/13 Abstract: Microscopes are fragile instruments that must be handle with extreme caution as they can produce high quality results when observing the smallest specimens on earth. A microscope must be properly cleaned before use and storage. The different objectives allow for a range of observations. At the highest objectives‚ the resolution can easily be lost which is why the oil immersion lens is used to minimize refraction. While
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