diaphragm aperture; the difference in lighting between adjacent areas of the specimen C. Describe your observations from fresh yogurt slide you prepared in Part III. While viewing under 4x magnification‚ you can see the presence of cocci and bacillus bacteria strains. After increasing the magnification to 10x‚ you can view these shapes more clearly‚ but it is not until 40x magnification that you can see the cooci exist in pairs and strands‚ in other words‚ diplococci and streptococci are present
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would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly with my results but Lysol was only 4mm more sustainable then Windex. Lysol with the biggest zone of inhibition of 28mm allowing this disinfectant to kill the most Bacillus subtilis bacteria. Still‚ Lysol was succeeded to work
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accounted by the slow movement of molecules that decreases enzyme and substrate collision. The difference in optimal temperature between the fungal and bacterial amylase may be due to the different environments that each enzyme is naturally found in. Bacillus licheniformis is found in a wider range of environments so its enzymes have to survive a higher range of temperatures. Where as Aspergillus oryzae is found in lower temperature habitats preventing its enzymes from tolerating a wider range of temperatures
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Morales‚ Marinel M. Dr. Annie Cu Gallardo BSE-Biological Sciences III-3 Microbiology (Lecture) Microbiology Written Report REPORT NO. 1 Prokaryotes: Bacteria Most of us have been conditioned to think of bacteria as invisible‚ potentially harmful little creatures. Actually‚ relatively few species of bacteria cause disease in humans‚ animals‚ plants‚ or any other organisms. In fact‚ all organisms made up of eukaryotic cells probably evolved from bacteria-like organisms‚ which were some
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Several cells majority Bacillus and cocci cells. D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so‚ describe them. Only perhaps that there was far more Cocci cells then Bacillus E. Describe the four main bacterial shapes 1. Cocci - Spherical or oval bacteria having one of several distinct arrangements based on their planes of division 2. Bacillus - Rod shaped bacteria. Bacilli all divide in one plane producing a bacillus‚ streptobacillus
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Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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a good bioterrorism agent. How is Anthrax Caused? Anthrax is caused by the bacterium[2] ‘Bacillus anthracis’ that is highly lethal in its virulent form. Anthrax most commonly occurs in wild and domestic animals‚ but it can also occur in humans when they are exposed to infected animals‚ tissue from infected animals‚ or high concentrations of anthrax spores.[3] Bacillus Anthracis Bacillus anthracis‚ the bacterium which causes anthrax‚ is rod-shaped and about 1 by 6 micrometres in size
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Biotechnology In environment Clean up Process - APPSC G1 Mains - Paper 4 - Section 2 - Unit 3 The term "Environment" is defined as our surroundings which includes the abiotic component (the non living) and biotic component (the living) around us. The abiotic environment includes water‚ air and soil while the biotic environment consists of all living organisms – plants‚ animals and microorganisms. Environmental pollution broadly refers to the presence of undesirable substances in the environment
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microscopes. Simple Microscopes Compound Microscopes Scanning Electron Microscope Transmission Electron Microscope #4. Identify these stains: (Functions and Reagents) Simple Stain- CULTURES: Staphylococcus epidermis slant Bacillus megaterium broth MATERIALS USED: Methylene blue‚ Distilled water‚ Slide‚ Inoculating Loop FUNCTION: To observe shape size‚ morphology‚ and arrangement. ---FROM SOLID: (slant) 1. Mark the smears on the underside. 2. Place 1 to
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Observing Bacteria Kelli Jo Simco Microbiology Due: 2/8/13 Abstract: Microscopes are fragile instruments that must be handle with extreme caution as they can produce high quality results when observing the smallest specimens on earth. A microscope must be properly cleaned before use and storage. The different objectives allow for a range of observations. At the highest objectives‚ the resolution can easily be lost which is why the oil immersion lens is used to minimize refraction. While
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