Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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Aseptic Technique & Culturing Microbes Purpose: To learn and employ aseptic technique and basic forms of culture media as well as become familiar with the basic requirements of microbial growth and the methods used to control microbial growth. Procedure: Obtained a small Styrofoam cooler placed two small light bulbs in side and observed temperature over 24 hours to ensure temperature could be maintained between 98-100 degrees. Using a 10% bleach solution I then cleaned my work area. Transferring
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media can be used to culture and identify bacteria. Some
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1: The Microbial World and You Learning Objectives Go Over First Lecture 1-1 List several ways in which microbes affect our lives. 1-2 Recognize the system of scientific nomenclature that uses two names: a genus and a specific epithet. 1-3 Differentiate the major characteristics of each group of microorganisms. 1-4 List the three domains. 1-5 Explain the importance of observations made by Hooke and van Leeuwenhoek. 1-6 Compare spontaneous generation and biogenesis. 1-7 Identify
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Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and macroscopic) ♣Immunological- serological analysis ♣Genotypic- genetic techniques Dr.T.V.Rao MD 2 Microbe Identification
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effective in killing bacteria‚ fungus‚ and viruses. Household bleach works quickly and is widely available at a low cost. The disadvantage is could irritates mucous membranes‚ the skin‚ and the airway. It also decomposes under heat or light and reacts readily with other chemicals. Bleach solutions begin to lose its effectiveness after 2 hours. You will need to make a fresh solution for each experiment. The advantage of the 70% alcohol mixtures is capable of killing most bacteria within 5 minutes of
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Anaerobic respiration in Bacteria Like humans and other animals‚ bacteria need to breathe. Anaerobic bacteria basically mean bacteria that respire anaerobically. All living cells need to respire in order to release energy for vital cell activities. Anaerobic respiration is respiration in the absence of oxygen‚ hence anaerobic bacteria does not breathe at all. The purpose of respiration is to provide the cell with the appropriate molecules for creating energy in the form of adenosine triphosphate
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Antibiotic Resistant Bacteria A better understanding of the use of antibiotics would help prevent the reality of antibiotic resistant bacteria evolving to the point of human extinction. Antibiotic resistance is a natural process‚ stronger bacteria survive and multiply. Even though antibiotic resistance is a natural process‚ it happens faster when antibiotics are used irresponsibly. Through use of media‚ personal responsibility and research‚ our species could help deter further antibiotic resistance
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