Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
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Lecture 14 Lecture Gene Complementation in Bacteria In order to perform tests for dominance or for complementation in bacteria we need a way to make the bacteria diploid for part of the chromosome. To do this we need to consider a different extrachromosomal element: Ori T The F plasmid (length 105 base pairs) Tra genes There are some special terms to describe the state of F in a cell: F– refers to a strain without any form of F‚ whereas F+ refers to a strain with an F plasmid. F‚
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1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a sample of different
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Cells Now we need to discuss CELLS. You’ve probably heard of cells‚ and may even know of a few‚ such as blood cells‚ nerve cells‚ or skin cells. What are these things called cells? Cells are the smallest living things. You’ll be learning a lot about cells in this course. For now‚ however‚ I’ll introduce you to just a few basic things about them. Cells are extremely tiny organisms that carry out all of the functions of life inside a thin layer of “skin” called (unimaginatively) the CELL MEMBRANE
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MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed‚ there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is
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health and is easily infectious with contaminated sustenance. E. coli is gram-negative bacilli tested in clinical settings from stool specimens. The tests detect the presence of disease causing pathogens of E. coli that produce Shiga toxins. E. coli bacteria commonly occur in nature and they are a necessary component of the digestive process in humans and most other mammals. Most strains of E. coli are harmless‚ but pathogenic E. coli can be responsible for inflammation of the stomach and intestines
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Antony R 9:00Am-11Am Unknown Number 94 Unknown Results Streptococcus salivarius The first step in my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram
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Conclusion Genetically modified organisms have their good and bad points. They can produce more food and provide many benefits to humans. But they can also be detrimental if not used properly. They can mix with non genetically modified crops and ruin a farmers business. Pesticides in plants can be a great benefit but they can damage the environment and be poisonous to the consumer. There is a lot of conflict over whether genetically modified organisms are good or bad for us and there is no way to
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a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria are unicellular microorganisms‚ with variable shapes and nutritional needs. They lack a distinct nucleus and occur singly or in chains or clusters and form distinct colonies. Bacteria are classified on the basis of many
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Prokaryotes: Bacteria Most of us have been conditioned to think of bacteria as invisible‚ potentially harmful little creatures. Actually‚ relatively few species of bacteria cause disease in humans‚ animals‚ plants‚ or any other organisms. In fact‚ all organisms made up of eukaryotic cells probably evolved from bacteria-like organisms‚ which were some of the earliest forms of life. In this chapter‚ we will learn how bacterial groups are differentiated from each other and how important bacteria are in
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