Formal lab report: Abstarct: The Purpose of this experiment was to perform Agrobacterium-mediated transformation in Wild type Arabidopsis thaliana Columbia by using somatic plant transformation method. The whole process lasted for over a period of 11 weeks and we were successful in getting transformed plants. Agrobacterium tumefaciens strain containing pMP90 (Ti-helper) plasmid and pCAMBIA1391 (T-DNA) plasmid was used for this plant transformation. pCAMBIA1391 plasmid was constructed by cloning Brassica
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Bacterial Energetics and Membranes Abstract The Mg2+/Ca2+ ATP synthase present in all bacterial membranes‚ particularly E. coli‚ couples ATP synthesis to the proton (H+) gradient produced by the ETC‚ a process known as oxidative phosphorylation. The gradient acts to power the ATPase‚ so that it may phosphorylate ADP to produce ATP. The reverse reaction of this process‚ or hydrolysis of ATP into ADP and Pi‚ may be used to observe ATPase activity when the resulting Pi is quantitatively measured
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English settlers ventured out to establish their claims over lands in the New World. Two principal areas they established were the English colonies of the Chesapeake and their counterparts at Massachusetts Bay. The English colonies and the Massachusetts Bay settlements were different economically and socially but similar religiously. The Chesapeake colonies were founded on a basis of mainly economic pursuits and were the principal exports of tobacco‚ whereas the Massachusetts area settlers were
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products; Scope Mouthwash (Scope)‚ LISTERINE® Antiseptic Mouthwash (Listerine)‚ Bactine Original First Aid Liquid (Bactine)‚ and Povidone-Iodine Solution‚ 10% Topical Microbicide Antiseptic (Iodine) are an appropriate antiseptic to can kill a sample bacterial contaminant found in the local environment‚ and if so‚ which is the most effective? The purpose of this experiment is to determine the antibacterial effectiveness of Scope‚ Listerine‚ Bactine‚ and Iodine in relation to each other. Scope‚ Listerine
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While each person chosen to live on the colony has to be smart‚ loyal‚ brave‚ compassionate‚ etcetera‚ I believe that each person also has to have unique skill sets that set them apart from the others. For example‚ while they each have the qualities listed above‚ perhaps once could be a leader‚ the other a scientist‚ another an artist‚ and so on. This way‚ the colony has a variety of characters that will lend their talents to the growth of the strongest colony possible. The first of the four people
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Enumeration of Bacterial Contamination in Hamburger Meat from Unknown Sources C March 6‚ 2012 The importance of bacterial enumeration has become even more apparent in recent years due to the increasing numbers of harmful bacteria found in meat products. This process is the key to understanding the populations of microorganisms that contaminate the food supply. Much of the bacteria in meat has been shown to be resistant to multiple drugs; so disease-causing microbes
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At the beginning of the semester‚ samples of bacteria were obtained from various sources. Two bacterial colonies were isolated from plates that were incubated in the lab and these bacteria became the basis for this project. The bacterial cultures were maintained and used for various physiological‚ cultural‚ and biochemical tests and observations over several weeks. The purpose of culturing bacteria over half the course of the semester was to learn various techniques used in microbiology labs and
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Growth Dynamics of E. coli in Varying Concentrations of Nutrient Broths‚ pH‚ and in the Presence of an Antibiotic Dvora Szego‚ Elysia Preston Darcy Kmiotek‚ Brian Libby Department of Biology Rensselaer Polytechnic Institute Troy‚ NY 12180 Abstract The purpose in this experiment of growth dynamics of E. coli in varying media was to determine which media produces the maximum number of cells per unit time. First a control was established for E. coli in a 1.0x nutrient broth. This was used to compare
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The Effects of Antibiotics on Bacterial Growth Biology II 1996 Bacteria are the most common and ancient microorganisms on earth. Most bacteria are microscopic‚ measuring 1 micron in length. However‚ colonies of bacteria grown in a laboratory petri dish can be seen with the unaided eye. There are many divisions and classifications of bacteria that assist in identifying them. The first two types of bacteria are archaebacteria and eubacteria. Both groups have common ancestors dating to more
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Economic‚ geographic‚ and social factors all contributed to the rise of importance for slaves in the southern colonies as their position in American society changed from 1607 and 1775. Slaves not only influenced Jamestown in 1607‚ but they influenced America‚ all the way into the American Revolution in 1775. Slavery ultimately flourished and aided economic triumph in the southern colonies. Slavery was not only a cheap source of labor in the Americas‚ but it was effective too‚ as slaves greatly
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