Prof. Kimberly Rutledge BIO 150 - Unknown Bacterium Identification March 26‚ 2014 Lap Report Unknown Number : #12 Name of Unknown : Pseudomona aerugninosa The identification of unknown bacteria is a time-honored part of microbiology courses. It will challenge my knowledge and skill in performing laboratory techniques and my ability to critically evaluate the information obtained from these techniques. The unknown bacterium tube number 12 was picked. According to the procedures that we have
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OBSERVATION OF BACTERIAL CELLS VIA MICROSCOPE In this experiment‚ we have learned several basic molecular biology techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained
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Cellulitis is a bacterial infection that affects the skin and the tissue underneath the skin. Although cellulitis can be caused by many types of bacteria‚ streptococcus and staphylococcus are the main bacteria that cause this condition. Cellulitis often develops near surgical wounds or ulcers. This condition causes the skin to become reddened and swollen. It also causes the area to feel tender and hot. Cellulitis can quickly spread to other areas of the body. It is not contagious. Cellulitis can
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Disease: Ergotism Name of Pathogen: Claviceps purpurea Body System affected: Lymphatic/Immune sytstem‚ Digestive‚ Circulatory Bacterial Morphology: They are fungi that resemble small mushrooms in which the perithecia are embedded in the capitate tip. Reservoir: grains (specifically rye) and grass contaminated with ergot Mode of Transmission: Ingestion Symptoms of Illness: Symptoms include constriction of arteries and veins‚ rapid‚ weak pulse‚ precordial distress or pain muscle pain
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Discussion After doing a series of different biochemical tests for the isolated organisim from the sputum sample‚ it was concluded that the unknown Gram positive organism was Streptococcus pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth
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Endocrine and renal ascites are some of the less common disorders. In case of Ascites ovarian cancer‚ the fluid contains freely-floating cancer cells separated from the cancerous growths. Reference Amadon MN‚ Arroyo V. Ascites and spontaneous bacterial peritonitis. In: Schiff ER‚ Sorrell MF‚ Maddrey WC‚ eds. Schiff’s Diseases of the Liver. 8th ed. Philadelphia‚ Pa:
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Necrotizing Fasciitis is a bacterial skin infection that is caused by one or more bacteria that enters one’s skin through a cut or wound. It can be fatal if not treated in time. Necrotizing Fasciitis is commonly known as the ‘flesh eating infection’ that occurs suddenly and spreads extremely fast. It corrodes the skin and the tissue beneath it. It can be caused by one or more bacteria‚ for example Streptococcus pyogenes‚ Kebsiella‚ Bacteroides and more. Approximately 700 hundred cases are recorded
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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Roni Añasco Lee Balmaceda Ian Leonio Polo Reyes Ficus stipulosa Miq. Linn (Balete) sap as an alternative material to produce environment friendly cups Review of Related Literature Collecting Sap For many years‚ the means to collect sap was to hang a bucket to the tree‚ and to wait for the sap to drip out. This process was very slow and ineffective. Later on they started a new method of sap collecting which was attaching a tube to each tree that would all lead to a central station. Scientists
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and identification of Bacterial isolates An enrichment culture technique was used for the isolation of bacteria responsible for biodegradation of phorate in soil. Screening of these bacterial species for phorate degradation in liquid cultures in our previous study (Jariyal et al.‚ 2014)‚ resulted in identification of bacterial species B. aerophilus strain Imbl 4.1 ‚ Brevibacterium frigoritolerans strain Imbl 2.1 and Pseudomonas fulva strain Imbl 5.1. However‚ these bacterial species‚ causing complete
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