Bacterial Morphology Part 1: Viewing Prepared Slides of Common Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining: Record your observations for each sample. Part 5: Indirect Staining: Examine the stained
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Chapter 1 THE PROBLEM AND ITS BACKGROUND Introduction The development of dyes to stain microorganisms was a significant advance in microbiology. Stains serve purposes such as it differentiate microorganisms from their surrounding environment and it allows detailed observation of microbial structures at high magnification. (http://inst.bact.wisc.edu/‚ © 2006-2013 Microbiology Laboratories) Gunasekaran (2005) defined staining as the method of artificially producing color in microorganisms to allow
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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bodies. The experiment conducted was to test how different temperatures effected the catabolizing of fungal and bacterial amylase‚ as well as the optimal temperature needed for the enzyme to correlate with the bacteria and fungi. The enzyme’s break down within the starch was observed through different temperatures and time periods. The Starch was placed in both the fungal and bacterial amylase where they were then placed on spot plates. Through the iodine test‚ it was concluded whether the breakdown
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(JOURNAL ARTICLE) By SITUMBEKO LIWELEYA (s213459531) Submitted in fulfilment of the requirements for the degree of BACHALOROUS TECHNOLOGIEA: BIOMEDICAL TECHNOLOGY At the At Nelson Mandela Metropolitan University Port Elizabeth‚ 2013. SUPERVISOR- PROFESSOR SMITH. N. Biotechnology Research International Journal Instruction Page Article Processing Charges Biotechnology Research International is an open access journal. Open access charges allow publishers to make the
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lab was conducted to determine the optimal temperature for bacterial and fungal Amylases and evaluate how temperature affects the catabolic rate of enzymes. Enzyme reaction rate was measured using an Iodine test in which drops of starch solution with either fungal or bacterial Amylase exposed to different temperatures were mixed with Iodine. Iodine is a dark blue color in the presence of starch and turns light yellow in its absence. Bacterial Amylase had an optimal temperature of 55°C‚ meaning that
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Some strains of Acetobacter produce a gelatinous membrane called pellicle or bacterial cellulose (BC) at the surface of liquid culture. BC is also called as “Nata de Coco”‚ a traditional fermented food in southeast Asia. Coconut-water is regarded as a waste from the copra process‚ the cost is low and easily to obtain. Although in these areas using mature coconut water to produce nata de coco obtained a good yield of pellicle was obtained‚ the shortage of coconut water is always affecting the stability
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corn‚ hops‚ yeast‚ antioxidants (ascorbic acid)‚ and propylene glycol as a stabiliser.[5] [edit]Gluten quantities Some proponents of gluten-free diets for general health or specific conditions‚ such as celiac disease and SIBOS (small intestine bacterial overgrowth syndrome)‚ advise beer consumers to drink Corona because of its low gluten quantities. Bottles of Corona Extra contains less than 20 ppm gluten‚ while other typical beers contain well over 2000 ppm.[6] The limit for gluten free is 200
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“DISCUSS THE STRATEGIES THAT ARE USED BY BACTERIAL PATHOGENS TO EVADE HOST ANTIBACTERIAL PEPTIDES AND PHAGOCYTES” The mammalian immune system has two major components; the innate and the adaptive immune system. The innate system is usually the first line of defence and it acts as a strong roadblock to invading bacterial pathogens through direct mechanisms such as inflammation‚ phagocytosis‚ complement system activation‚ antibacterial peptides and indirectly by activating adaptive response through
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Effect of varying Temperatures on Enzymatic Activity of Bacterial and Fungal Amylase and hydrolysis of Starch Abstract This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added
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