Chapter 1 THE PROBLEM AND ITS BACKGROUND Introduction The development of dyes to stain microorganisms was a significant advance in microbiology. Stains serve purposes such as it differentiate microorganisms from their surrounding environment and it allows detailed observation of microbial structures at high magnification. (http://inst.bact.wisc.edu/‚ © 2006-2013 Microbiology Laboratories) Gunasekaran (2005) defined staining as the method of artificially producing color in microorganisms to allow
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Lab Report Microbiology Introduction Bacteria can be found almost anywhere. For human life‚ some help us‚ some hurt us‚ and others are neutral. It is now known that good bacteria‚ or normal microflora‚ can reach 1014 microbial cells. This is far more than the 1013 cells that make up the human body (Tannock‚ 1995). The total number of bacteria on Earth is estimated to be around 4-6 x 1030 (Horner-Devine‚ 2004). It is important to know the extent of bacteria‚ how they live‚ and how they are
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served as a thickening for soups and sauces‚ in jellies and ice cream‚ in cosmetics‚ for clarifying beverages‚ and for sizing fabrics. Agar is also used in medicines to promote peristalsis and relieve constipation and is resistant to a breakdown by bacterial enzymes. Sodium carbonate‚ Na2CO3‚ also known as washing soda or soda ash‚ is a sodium salt of carbonic acid. It can be extracted from the ashes of many plants and has a cooling alkaline taste. It is one of the most basic industrial chemicals.
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Blood Cynthia Alonzo‚ M.S. Version 42-0249-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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a differential stain. Mycobacterium and some Nocardia species are considered acid fast because‚ during the acid-fast procedure‚ they are able to retain the primary dye even when decolorized by a powerful solvent known as acid alcohol. Most other bacterial genera are easily decolorized by acid alcohol. Ziehl/Neelsen Acid-Fast Staining Procedure In later years‚ Ehrlich’s technique was improved upon by two microbiologists‚ Ziehl and Neelsen. Like Ehrlich‚ Ziehl and Neelsen’s procedure requires heat
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Lab Questions: Lab #4 * Why are the chloroplasts traveling along the outer perimeter of the Elodea cell? The chloroplast are traveling along the outer perimeter to help move the vital nutrients thought the cells and convert them to substances used in the cell of the elodea cell. * What is the typical size difference between animal cells and bacterial cells? One of the main difference between an animal cell and a bacterial cell is a bacterial cell contains a plasmid‚ a ring of DNA‚ opposed
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African Diaspora and the World After reading the short story “Diaspora” by Joanne Hyppolite‚ I got a vivid understanding of how diaspora can have an effect on one’s identity. Over the past month we have studied many theories and concepts concerning race‚ gender‚ and politics. There are three theories that capture the essence of Joanne Hyppolite’s worldview as a Haitian growing up in America: intersectionality‚ identity‚ and diaspora. Individuals oftentimes experience the theory of Intersectionality
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as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria on it to see if the bacteria obtained is gram positive or gram negative. My hypothesis is there will be a lot of bacterial growth on all of the plate. Materials and Methods -Petri dish containing nutrient agar
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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