Antibiotics Chang Liu Instructor: Prof. Jasson Sello Department of Chemistry‚ Brown University Providence 02906‚ RI Tetracyclines are the first broad-spectrum antibiotic to be applied to clinic use. Nevertheless‚ the increasing incidence of bacterial resistance of tetracyclines led to a series of studies on the development of semisynthetic tetracyclines to circumvent the resistant organisms. In order to better design the structures of tetracycline derivatives‚ research on the action mode
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function of iodine when used in gram staining? (2 marks) Its function is to form large crystals with crystal violet so that they stick to the bacterial cell wall and are not washed away easily. 4. Attach photos on JPEG format showing a gram positive and gram negative bacterial sample showing their gram staining reaction. Give the name of the bacterial cells‚ shapes and arrangement. (9 marks) Bacillus cereus: Rod shaped and chain arrangement‚ Gram positive http://aapredbook.aappublications
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to redness. 6. Remove the tube cap with the fingers of the hand holding the loop. Flame the tube mouth. 8. Touch the inoculating loop to the inside of the tube to make sure it is not so hot that it will distort the bacterial cells; then pick up a pinhead size sample of the bacterial growth without digging into the agar. COMPLEX STAINING Reflame the tube mouth‚ replace the can‚ and put the tube back in the holder. 10. Disperse the bacteria on the loop in the drop of water on the slide and spread
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Prof. Kimberly Rutledge BIO 150 - Unknown Bacterium Identification March 26‚ 2014 Lap Report Unknown Number : #12 Name of Unknown : Pseudomona aerugninosa The identification of unknown bacteria is a time-honored part of microbiology courses. It will challenge my knowledge and skill in performing laboratory techniques and my ability to critically evaluate the information obtained from these techniques. The unknown bacterium tube number 12 was picked. According to the procedures that we have
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OBSERVATION OF BACTERIAL CELLS VIA MICROSCOPE In this experiment‚ we have learned several basic molecular biology techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained
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Cellulitis is a bacterial infection that affects the skin and the tissue underneath the skin. Although cellulitis can be caused by many types of bacteria‚ streptococcus and staphylococcus are the main bacteria that cause this condition. Cellulitis often develops near surgical wounds or ulcers. This condition causes the skin to become reddened and swollen. It also causes the area to feel tender and hot. Cellulitis can quickly spread to other areas of the body. It is not contagious. Cellulitis can
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Disease: Ergotism Name of Pathogen: Claviceps purpurea Body System affected: Lymphatic/Immune sytstem‚ Digestive‚ Circulatory Bacterial Morphology: They are fungi that resemble small mushrooms in which the perithecia are embedded in the capitate tip. Reservoir: grains (specifically rye) and grass contaminated with ergot Mode of Transmission: Ingestion Symptoms of Illness: Symptoms include constriction of arteries and veins‚ rapid‚ weak pulse‚ precordial distress or pain muscle pain
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Discussion After doing a series of different biochemical tests for the isolated organisim from the sputum sample‚ it was concluded that the unknown Gram positive organism was Streptococcus pyogenes. The sputum sample was first inoculated in the agar plates MAC‚ SBAP‚ CHOC and also a direct gram stain of the sample was performed. After a day of incubation‚ the MAC agar gave a negative result‚ therefore it is not a Gram-negative bacteria and non-lactose fermenting. The SBAP and CHOC showed growth
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Endocrine and renal ascites are some of the less common disorders. In case of Ascites ovarian cancer‚ the fluid contains freely-floating cancer cells separated from the cancerous growths. Reference Amadon MN‚ Arroyo V. Ascites and spontaneous bacterial peritonitis. In: Schiff ER‚ Sorrell MF‚ Maddrey WC‚ eds. Schiff’s Diseases of the Liver. 8th ed. Philadelphia‚ Pa:
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Necrotizing Fasciitis is a bacterial skin infection that is caused by one or more bacteria that enters one’s skin through a cut or wound. It can be fatal if not treated in time. Necrotizing Fasciitis is commonly known as the ‘flesh eating infection’ that occurs suddenly and spreads extremely fast. It corrodes the skin and the tissue beneath it. It can be caused by one or more bacteria‚ for example Streptococcus pyogenes‚ Kebsiella‚ Bacteroides and more. Approximately 700 hundred cases are recorded
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