of my microbiology class at Brunswick Community College. This journey started out with a media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with me on the journey to the unknown. As I recall from earlier that
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Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
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calculated the growth curve and it was 1.2. Using this I found the doubling time which was 34s. Introduction Bacteria grows by binary fission. One bacteria becomes two‚ two bacteria becomes four bacteria and it so goes on. A lot of changes occur in the bacterial cell during growth and due to this reason it is difficult to measure growth quantitatively. Growth is therefore usually measure by changing one or two easily measure parameters which will ignore the complicated cellular change. Therefore different
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Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
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Fighting Bacterial Growth The purpose of this lab was to determine the effectiveness of antiseptics‚ disinfectants‚ and antibiotics on bacteria. The hypothesis was that if bleach was used‚ it would be the most effective because bleach is commonly used to clean and disinfect various things. The variables that were tested were antibacterial soap and Scope mouthwash for the antiseptics; bleach and ammonia for the disinfectant; and Cipro‚ erythromycin‚ and tetracycline for the antibiotics. All of these
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into three different analysis; microscopy‚ soil microbiology and bacterial growth. The main aim of laboratory work with Escherichia coli and soil sample was to introduce students to bacterial growth in pure culture and soil microbial flora. The experiment of bacterial growth in pure culture using optical density measurement‚ plate count and DAPI direct count‚ showed that Plate Spread method was more precise to understand bacterial population growth tendency and counting generation time. During
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the alternate type yeast. This signals the two cells to join via fusion 1. The process by which the signal on the surface of the cell is converted to a series of steps by the cell in response is called a signal transduction pathway ii. Signal transduction pathways are very similar in yeast and in complex multicellular organisms 2. This leads scientists to believe that this pathway evolved first in ancient prokaryotes b. Local and long distance signaling
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immediate threat to human health. [1] In this article we explore contamination from this particular strain of E. coli bacteria (O157:H7) and enteric viruses‚ their potential threats and how to test the water for such contamination. Bacterial Contamination Bacterial contamination of water is usually in the form of coliform bacteria. Coliform bacteria live in soil/vegetation and in the gastrointestinal tract of animals and humans. They thrive in warm‚ wet and dark places.
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veterinarian immediately. Contagious bovine pleuropneumonia is not found in the United States. Suspicion of disease requires immediate attention. Animals are exposed to the CBPP bacteria by aerosol from the cough of infected cattle. Once inhaled‚ the bacterial infects the lungs of the animal. Another route of spread is by direct contact with
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Chapter 15 Signal Transduction 1) Endocrine‚ paracrine ‚ autocrine signaling‚ and cell-cell contact (Fig. 15-2). Endocrine signaling is long distance signaling. An example would be pancreatic cells secreting insulin. Paracrine signaling is for close proximity. An example would be a nerve cell releasing neurotransmitters. In autocrine signaling the cell that produces the ligand also contains the receptor for that ligand. This is how cancer cells work. In signaling by plasma membrane attached
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