Lab Report (Scientific Paper) 2: Bacterial Transformation;DNA Extraction Part I & II:Total Genomic Extraction & Plasmid Extraction;Electrophoresis By:Chris Foster Abstract: We conducted three experiments that included a Bacterial Transformation‚ a two process DNA extraction‚ and a final procedure using gel electrophoresis. The Bacterial Transformation lab was performed to prepare the plasmid into a bacteria and to use that bacteria to amplify the plasmid in order to make large quantities
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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Luria Broth. Once again‚ one will contain plasmid and one will not. The research question for this experiment is: What is the difference in the transformation efficiencies between pFLO and pBLU. Before completing this lab‚ a first trial was done following the same procedure below but instead of pFLO‚ pBLU was used. It is believed that the transformations efficiencies should be similar due to the fact that both plasmids are
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Bacterial Diversity Project John FreesackSection A24 Kim Daffer‚ John Chang September 23‚ 2012 Introduction: Bacteria are everywhere. Some can be seen with the naked eye and some require a microscope but how do we distinguish one kind of bacteria from another? To answer this question‚ we were required to complete three bacterial labs that helped us to understand what microorganisms are and how to identify and classify them. Thus‚ the main purpose of this project is to identify our unknown microorganisms
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Introduction: Bacteria need specific living conditions to survive. The ideal conditions for bacterial growth is a warm‚ dark‚ and damp environment. This provides the maximum effectiveness for bacterial growth. The areas in our school that contain the most bacteria would be the water fountains because the bacteria from our mouths drop off on to it when we drink from it‚ and because water fountains are not regularly washed in comparison to weight equipment. Water fountains also provide a warm damp
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Eucalyptus and Colloidal Silver. Obtain two nutrient agar plates and using a permanent marker draw four quadrants on the bottom of each agar plate. Using a sterile pipet transfer 250 ml of E. coli broth to the middle of each petri dish and evenly spread bacterial culture around the agar plate. Cover and allow the culture to soak into agar for at east 15 minutes. Using sterile forceps‚ carefully place one filter disk from designated sample into the middle of each
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Enumeration of Bacterial Contamination in Hamburger Meat from Unknown Sources C March 6‚ 2012 The importance of bacterial enumeration has become even more apparent in recent years due to the increasing numbers of harmful bacteria found in meat products. This process is the key to understanding the populations of microorganisms that contaminate the food supply. Much of the bacteria in meat has been shown to be resistant to multiple drugs; so disease-causing microbes
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Title of the lab: Transformation : Bacterial Genetics Purpose of the lab: The pupose of the lab was to transfor a bacterial E. Coli by using the green flurescent protein from the jellyfish. Another important that was fferdone by making the cell competency‚ meaning that it will be able to take on additional DNA. This was done when the plasma was added. Materials: 1. 37 o C water bath 2. Ice 3. Sterile transfer pipette 4. Foam tube rack 5. Transformation solution (CaCl2) 6. pGLO plasmid 7. Sterile
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Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
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Malak Zomrawi 4/9/15 Bacterial Transformation I. Abstract In the lab‚ the purpose is to see if we could move genes using plasmid. As well as getting better understand of transformation methods using shock wave. To see the effects five trays are being used containing LB nutrient broth. The results showed that the LB‚ ampicillin‚ and arabinose with a positive pGLO had the most amount of growth compared to the other four trays. Although when there is arabinose there is no fluorescence‚ fluorescence
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