The purpose of this experiment was understand the process of transformation and the effects on gene expression. The pGLO(-) culture had growth on the LB medium‚ while the LB amp and LB amp + ara mediums had no growth. It was expected that the LB medium had growth on the plate because it served as a control. The LB amp and LB amp + ara had no growth or glow under UV light because they were not successfully transformed and still contained the antibiotic ampicillin that prevented the growth of E. coli
Premium Escherichia coli Bacteria DNA
Transformation is the genetic alteration of a cell‚ resulting from the intake of exogenous genetic material through the surrounding cell membrane. The purpose of this lab was to determine transformation of bacteria by testing the effect of P Vib plasmid of E. coli MM294‚ and how the color of the E. coli bacteria changes. In this lab‚ two small test tubes were given calcium chloride‚ E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked
Premium
Modeling bacterial growth is important in maximizing the efficiencies of biological processes. Although there are many different methods of modeling bacterial growth‚ this experiment focuses on the Monod equations. However‚ in order to use the Monod equations‚ the maximum growth rate and Monod constant must be found. Here we show how the maximum growth rate and Monod constants can be obtained for Escherichia coli using M9 media in a bioreactor at 37 °C and 500 RPM. The maximum growth rate is also
Premium DNA Bacteria Gene
Title: Chapter 7: Bacterial Growth Purpose: The purpose of this experiment is to observe the bacterial growth of Escherichia coli under various conditions. Physical factors and nutritional requirements determine the overall concentration of the bacteria. Along with the use of a spectrophotometer and the technique of serial dilution‚ countable colonies can be obtained. Results are plotted on a semi-log graph in order to observe the different growth curves corresponding to optical density (cell
Premium Bacteria Escherichia coli Microbiology
E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation of E. Coli
Premium DNA Plasmid Gene expression
Transformation of Bacterial Cells with Plasmid DNA Introduction: Transformation refers to the process in which the cell integrates foreign DNA to its genetic code‚ meaning it takes the genes and incorporates them into the cell’s current DNA. Cells that can do this naturally‚ most commonly bacteria and archea‚ are known as competent. The bacteria E. coli do not have high transformation competence under normal conditions‚ but can be manipulated to produce better results using
Free Bacteria Antibiotic resistance Plasmid
Study of Bacterial Growth and Resistance Level to Certain Antibiotics INTRODUCTION Escherichia coli—better known as E. coli—is a gram negative‚ rod shaped bacteria. It is relatively harmless‚ but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as
Premium Bacteria Bacillus Antibiotic resistance
Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can
Premium Bacteria DNA Gene
inserted into the E.Coli. The purpose of the lab is to see if we can make the E.Coli glow and resistant to ampicillin. In the lab we were transforming a north american jellyfish by the name Aequorea victoria to produce GFP‚ which is a fluorescent protein which causes them to glow green only if it has its friend arabinose sugar “ARA” and if it’s under an ultraviolet light . We are expecting to see the bacteria grow and glow when the genetic transformation is completed. Throughout the few days the
Premium DNA Gene Genetics
Abstract: The topic of this research involved the occurrence of genetic transformation in bacteria (E. Coli). More specifically‚ a previously prepared pGLO plasmid--which consisted of the gene to be cloned--was used to transform non-pathogenic bacteria. The pGLO plasmid contained a gene for the Green Fluorescent Protein (GFP) from a bioluminescent jellyfish and a gene for resistance to ampicillin‚ an antibiotic. Essentially‚ we wanted to determine the conditions of the bacteria that would glow.
Premium Bacteria Plasmid DNA