Lab 5: Bacteriophage 5 February 2013 Introduction: A bacteriophage is essentially a virus that specifically infects bacteria. A bacterial cell infection progresses in much the same way as a eukaryotic cell. A plaque forms when a bacterial cells growing on soft agar burst from the viral infection and appears like a hole in the agar. Each plaque is created by the progeny of an individual phage and can thus be counted to determine the number of phage particles in a sample. The purpose of this
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Bacteriophage are readily available in soil and marine communities. The purpose of this lab was to investigate the bacteriophage (If present in sample collected) by testing the phage on a specific strain of bacteria and to observe its characteristics. This same approach can be used when testing for resistance evolution within bacterial strains. Our hypothesis states that bacteriophage will not be present within our sample from East Lake. With the use of a phage buffer and soft agar‚ it provides an
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A bacteriophage (informally‚ phage) is a virus that infects and replicates within bacteria. The term is derived from ’bacteria’ and the Greek φαγεῖν phagein "to devour". Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome‚ and may have relatively simple or elaborate structures. Their genomes may encode as few as four genes‚ and as many as hundreds of genes. Phage replicate within bacteria following the injection of their genome into the cytoplasm. Bacteriophage are among
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Bacteriophage A bacteriograph is any of a group of viruses that infect specific bacteria‚ usually causing their disintegration or dissolution. They are made of an outer protein coat or capsid that encloses the genetic material. They inject their genetic material into the bacterium following infection. When the strain is viruilent‚ all the synthesis of the host’s DNA‚ RNA and proteins ceases. The phage genome is then used to direct the synthesis of
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Microbiology Lab Report II Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior
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Life Cycle of Bacteriophage. Bacteriophage: Bacteria eating virus is called bacteriophage. Life Cycles: There are 2 types of lifecycles that occur in the bacteriophage: 1) Lytic Cycle 2) Lysogenic Cycle 1) Lytic Cycle: In lytic cycle‚ virus that is the bacteriophage causes lysis of the host cell. It is virulent phage. 2) Lysogenic Cycle: In lysogenic cycle‚ the bacteriophage does not cause lysis
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Introduction A bacteriophage is a virus that infects bacteria. A type of bacteriophage is bacteriophage lambda. This specific bacteriophage infects E. Coli. It is composed of protein and double stranded DNA ¡VdsDNA). The DNA of the phage contains around 50‚000 base pairs and codes for 50 proteins. At both ends of the DNA of the phage‚ there are cohesive ends‚ which are composed of 12 nucleotides. Both ends compliment each other‚ which makes the DNA circular once together. This circular DNA
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The debate of whether bacteriophages could replace antibiotics is one of the many health issues to be explored in this essay. Bacteriophages are viruses that specifically target bacteria in your body to destroy it. Many scientists believe that bacteriophages will replace antibiotics in the fight against bacterial/diseases‚ although ideal‚ scientist do not know the long term effects of using this mechanism of defense. More research is needed and thus the push for bacteriophage usage may be reluctant
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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