Hypothesis: If pH is increased or decreased past the enzyme’s optimum pH‚ the number of products produced by the enzyme will decrease because the enzyme will become denatured. Variables: The Independent variable is the pH of the environment. The uncertainty of pH is ± 1. pH is a unitless value. The Dependent variable is the number of products produced. The uncertainty of this this measurement is ± 1 product. In order for this experiment to be controlled‚ many variable were identified and held constant
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Assessment: Answer the following item with the appropriate response. 1. What is a mole ratio? 2. What piece of information is needed to convert grams of a compound to moles of the same compound? 3. Phosphine (PH3) can be prepared by the hydrolysis of calcium phosphide‚ Ca3P2: Ca3P2 + 6 H2O 3 Ca (OH)2 + 2 PH3 a) One mole of Ca3P2 produces 2 mol of PH3. b) One gram of Ca3P2 produces 2 g of PH3. c) 3 moles of Ca(OH)2 are produced for each 2 mol of PH3 produced
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E XPE RIME NT 4 . 5 Reactions of acids Aim To investigate and compare some reactions of a strong acid‚ hydrochloric acid‚ and a weak acid‚ ethanoic acid (common name‚ acetic acid) Equipment Dropper bottles containing: • 0.1 M hydrochloric acid‚ HCl • 0.1 M ethanoic acid (acetic acid)‚ CH3COOH • 0.1 M sodium hydroxide‚ NaOH • 1 M hydrochloric acid‚ HCl • 1 M ethanoic acid (acetic acid)‚ CH3COOH • universal indicator solution • limewater (calcium hydroxide‚ Ca(OH)2) Marble chips (calcium carbonate
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To identify an unknown sample‚ there were several steps that helped determine the molecular structure and the name of the sample. When Belle #9 (unknown) was given to me‚ it’s physical state and color was recorded on the data sheet. Since the sample was a pure liquid‚ there was no need for distillation. Next‚ a solubility test was given. When one drop of the unknown liquid was added to about 2 mL of water‚ the sample was tested to be soluble due to the dissolubility between the two compounds. By
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The Effects of varying p-Nitrophenyl Phosphate concentrations on Alkaline Phosphatase Activity Nathan Overholt Louis Settembrino March 5‚ 2002 Abstract: The enzyme Alkaline Phosphatase was set up in solutions of varying p-nitrophenyl phosphate concentrations. A fixed time assay was used to find the Michaelis constant and Vmax. All solutions were incubated at 30 C° for 30 mins. Absorbances were measured at 405 nm‚ and the Km was found to be 0.002 M (pNPP) and the Vmax to be 0.433 A/min. Introduction:
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The full strength 1M HCl acid had a pH level of 3.12 (Table 3.1). Sample A2 to A4 remained in the pH 3 level‚ while sample A5 and A6 had a pH range of 4 (Table 3.1). The pH level for 1M HCl dilutions slightly increased between each sample (Table 3.1). The diluted solutions of 1 M NaOH showed a few unexpected results. The original solution of 1 M NaOH was at a pH level of 11.50 (Table 3.1). However‚ the pH of sample B2 increased to 12.81 (Table 3.1). The remaining samples had a decreasing pH pattern
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The purpose of this lab is to gain experience because an Iodometric titration was preformed and to determine the molarity and mass percent of an analysts in an commercial product. In this experiment my partner and I prepared 500mL of 0.07M sodium thiosulfate pentahydrate. As well‚ we added 0.05 grams of sodium bicarbonate and mixed it together with DI water. We got 60 mL of sodium thiosulfate solution in a clean 100-mL beaker. Then filled the buret to just above the 0 mL mark with sodium thiosulfate
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Purpose: The purpose of this experiment is to analyze four drinking water samples from different places using four of the standard drinking water tests required by the EPA. The experiment will be performed by a four person group‚ with each person providing a different water sample. The four tests to be performed are: pH‚ conductivity‚ phenolphthalein and total alkalinity‚ and total and non-carbonate hardness. Data from these four tests will be gathered and analyzed‚ drawing conclusions “about
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Acid Rain and its Effects On Our Aquatic Ecosystems In the past century‚ one of the greatest threats to North America ’s aquatic ecosystem has been the widespread acidification of hundreds of thousands of waterways. Acid rain has effected plant and animal life within aquatic ecosystems‚ as well as microbiologic activity by affecting the rates of decomposition and the accumulation of organic matter. What causes this poisonous rain‚ and what can be done to improve North America ’s water quality
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Experiment #04: Synthesis of Salicylic Acid from Wintergreen Oil I. Introduction and Purpose Until 1874 commercial salicylic acid was synthesized entirely from natural wintergreen oil. In this experiment the salicylic acid is reproduced using methyl salicylate‚ the major constituent of wintergreen oil and later compared to salicylic acid made from benzene. The purpose of this lab is to determine the difference between salicylic acid made from methyl salicylate and salicylic acid made from benzene
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