BCH 369L–Biochemistry Laboratory Fall 2016 Module C Orlando Martinez uteid: olm298 Introduction The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin
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Lab #5: Refraction of Light Theory: Refraction can be defined as the bending of a wave when it enters a medium which causes it to have some reduced speed. In terms of light‚ refraction occurs when the ray passes through some medium which slows its speed; such as water or glass. In this instance the ray tends to bend towards the normal of the medium. The amount of bending or refraction which occurs can be calculated using Snell’s Law (). Objective: To measure the index of refraction of Lucite
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Lab: investigating hooked law with springs Purpose: to find spring constants of different springs using the slope of a graph of change in heights vs. the weight force. Also‚ to be able to understand how spring constants change when you add springs in a series or paralle Pre lab predictions: We predicted that the graph of gravitational force (mg) as a function of stretch (delta x) would look like Data: Spring #1: y = 8.2941x + 0.0685 This table represents the different distances that
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of the specialized underlying structures of these life-forms. In order for us to appreciate these special adaptation‚ we first need to know how a typical plant or an animal cell organelle behaves in different water and solute concentrations. In this lab‚ we will determine the effects of hypertonic‚ isotonic and hypotonic solutions on plant and animal cells. In general when an animals cell’s placed in hypertonic solution it shrivels; a plant cell on the other hand undergoes plasmolysis. When an animal
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Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
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Lab 5 The Diffraction Grating Chinua McDonald Objective: To measure the wavelength of light with a diffraction grating. Theory: The two types of diffraction gratings are the transmission and reflection gratings. They are made by ruling on a piece of glass or metal a number of evenly spaced lines with a fine diamond point. Diffraction phenomena can be analyzed in terms of Huygens’ principle‚ according to which every point on the wave front of a wave should be considered as a source
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indicated by a very pale pink color. To calculate the molarity of NaOH‚ the following equation was used MNaOH x VNaOH = MKHP x VKHP therefore the molarity was .125 M. INTRODUCTION This lab experiment covers the preparation of standard solution and the acid/base titration. The first part of the lab is to prepare a standard solution of Potassium hydrogen per. A standard solution is a solution of known concentration‚ in which it is prepared using exacting techniques to make sure that the molarity
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Moment of Inertia and Rotational Motion Garret Hebert PHY 2311 Tues 1:00 garret.hebert@hindscc.edu Abstract: During this lab we will study what rotational Inertia is and how different shapes of masses and different masses behave inertially when compared to each other. We will specifically study the differences of inertia between a disk and a ring. We will use increasing forces to induce angular acceleration of both a disk and a ring of a certain mass. We will then then measure the differences
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Introduction Chemical kinetics is the study of reaction rates. A reaction rate is the speed of the change in either reactants or products over a period of time. General kinetic rate equation is: Where [A] and [B] are the concentration of the species in the reaction. The variable k is the rate constant‚ which is a function of time and catalyst presence. The variables m and n are the order of reaction for their respective species concentration. The higher the value of the reaction order the
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