BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
Premium DNA Escherichia coli Bacteria
T G M k h da — d c m m n p f a Physical Constants Acceleration due to gravity Universal gravitational constant Electron charge Speed of light Boltzmann’s constant Planck’s constant Electron rest mass Proton rest mass Neutron rest mass Coulomb’s law constant Permittivity of free space Permeability of free space Astronomical and Earth data Radius of the Earth equatorial polar average Mass of the Earth the Moon the Sun Average distance of the Earth from the Sun Average distance of the Moon from the
Premium Measurement
Ltd Assignment Question Part B John Weston Foakes v Julia Beer (1884) UKHL 1‚ (1881-85) All ER Rep 106‚ (1884) 9 App Cas 605; 54 LJQB 130; 51 LT 833; 33 WR 233 http://www.bailii.org/uk/cases/UKHL/1884/1.htmlAtlas Express v Kafco (Importers & Distributors ) Ltd (1989) QB 833 http://en.wikipedia.org/wiki/Atlas_Express_Ltd_v_KafcoAmalgamated Investment Co v Texas Bank (1982) Q.B http://www.bailii.org/ew/cases/EWCA/Civ/1965/3.htmlBusiness Law‚ by Robert W. EmersonPublished by Barron ’s Educational
Premium Contract
Supremacy of EU law The legal doctrine of supremacy of EU law means that EU labour law takes precedence over domestic labour law. The creation of a new legal order of EU law and its supremacy means that EU institutions may create rules affecting employment and industrial relations‚ even where some Member States oppose such rules and vote against them in those EU institutions‚ provided that a voting procedure based on a majority rule applies to that specific field. Where adopted‚ these rules must
Premium European Union
Kristen Sierman Lab Report: Experiment #7 Group #4 October 16th‚ 2012 Forces on a Pulley System A. Statement of the Problem The purpose of this lab was to address the following questions: how does the acceleration of a pulley system depend on the mass of the driving objects? And how does the acceleration of a pulley system depend on the mass of the driven object? To achieve this‚ a dynamic track was set up with a string attached to a cart. The string was part of a pulley system (the pulley
Premium Force Classical mechanics Mass
Abstract: This report will show the acquired understanding of the refrigeration cycle by using first and second laws of thermodynamics. In order to analyze this system several assumptions where made such like an isentropic process at the compressor an isenthalpic expansion in the throttling valve. Diagrams will be provided to depict these thermodynamic processes in addition to computing the heat transferred to the system and the work input to the compressor. Table of Contents Abstract .
Premium Heat Thermodynamics Heat transfer
An Investigation of Ohm’s Law Stephen McGill (22044566) Results from test using 25Ω Resistor: Voltage(Volts) | Current(mA) | Resistance(Ohms) | 0 | 0 | 0 | 0.57 | 20 | 28.5 | 1.01 | 40 | 25.25 | 1.49 | 60 | 24.83 | 2.01 | 80 | 25.12 | 2.51 | 100 | 25.1 | 3.0 | 120 | 25.0 | 3.51 | 140 | 25.0 | 4.0 | 160 | 25.0 | 4.5 | 180 | 25.0 | 5.03 | 210 | 23.95 | 5.52 | 230 | 24.0 | 6.02 | 250 | 24.08 | 6.51 | 270 | 24.1 | 7.0 | 290 | 24.13 | 7.5 |
Premium Electrical resistance Ohm's law Voltage
Back Laboratory Program SAFETY IN THE CHEMISTRY LABORATORY 786 Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic
Premium Water Chemistry Drinking water
Lab Report 1 Introduction: Proper chemical formulas entitle many challenges such as the Law of Multiple proportions that states that there may be more than one plausible mole ratio for the elements in that compound. However if we determine the mass of each element in the compound we will be able to get the true chemical formula. In this experiment‚ we used the law of definite proportions to find the chemical formula for a hydrated compound containing copper‚ chlorine‚ and water molecules
Premium Chlorine Water Ion
Purpose and Method: The purpose of this experiment was to understand Boyle’s Law. In the experiment the pressure in the system under constant temperature and mass was used to confirm if the laws are true. Boyles law relates pressure and volume while all other factors are consistent and states: for a fixed amount of gas kept at constant temp‚ the product of the pressure of the gas and its volume will remain constant if either quantity is changed‚ or where k is constant. The experiment consisted
Premium Pressure Volume Thermodynamics