Colorimetry Introduction Absorption Spectroscopic methods of analysis rank among the most widespread and powerful tools for quantitative analysis. The use of a spectrophotometer to determine the extent of absorption of various wavelengths of visible light by a given solution is commonly known as colorimetry. This method is used to determine concentrations of various chemicals which can give colours either directly or after addition of some other chemicals. As stated by the Beer-Lambert Law
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Determining the Concentration of a solution: Beer’s Law Objective In this lab of Determining the concentration of a unknown solution: Beers Law. We determined the concentration of a unknown CuSO4 solution by measuring its absorbance with the colorimeter. With all the calculations we were able to solve the linear regression Equation of absorbance vs. concentration and the alternate method. Materials Vernier LabPro or CBL 2 interface .40 M CuSO4 solution Computer or handheld
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SPECTROPHOTOMETRY Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength It involves with the use of a spectrophotometer. A spectrophotometer is a photometer that can measure intensity as a function of the light source wavelength. A spectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions‚ transparent or opaque solids‚ such as polished glass‚ or gases. Principles
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experiment is to apply Beer’s Law by analyzing samples provided by Q laboratory to determine their absorbance and prepare a Beer’s law plot. Molarity of these samples was also calculated to determine concentration and percent error rate. Students also analyzed the concentration of blue dye #1 to determine the concentration of blue dye #1 in a commercial blue dye drink. Procedure Exercise #1 Step #1: Convert %T (Table 1) to absorbance and prepare a Beer’s law plot using the data. Step #2:
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Chemistry Beer’s Law & Colorimetry Submitted by Monika Rebalska Date Submitted: 3/7/2015 Date Performed: 3/5/2015 Lab Section: Chem-180 Course Instructor: Cynthia Spencer Purpose The purpose of this experiment was to construct a Beer’s Law Plot‚ determine the concentration of a sample using the Beer’s law plot‚ and
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Absorption Spectra and the Beer-Lambert Law. ɛ Purpose : Understanding of Beer –Lambert Law Introduction : When light passes through the colour solution ‚ the molecules of the solution absorb the quantity of light at a particular wavelength . The amount of light which is absorbed by solution depend on two things. 1. The length of the pathway of light 2.Concentration of colour solution. These are all explained by the Beer-Lambert Law. Absorbance (A) = ɛcl
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Beer-Lambert Law Gabe Garrison & Paige England Buffalo High School Abstract In this lab‚ the Beer-Lambert Law is tested by using a spectrophotometer. This is a quick and easier way to determine the concentration of a solution. Keywords: Beer-Lambert Law‚ Spectrophotometer‚ concentration Beer-Lambert Law The Beer-Lambert-Law states that
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Title: Mitosis and Meiosis Abstract: There are two types of nuclear division‚ mitosis and meiosis. Mitosis is usually used for the growth and replacement of somotaic cells‚ while meiosis produces the gametes or spores used in an organism’s reproduction. Hypothesis: Mitosis occurs in whitefish blastula and onion root tip‚ and it is easily observable. Meiosis and crossing over occurs in the production of gametes and spores. Materials: This lab required prepared slides of whitefish blastula
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Beer-Lambert Law Relationship Between Molar Concentration and Absorbance Solution colour results from the absorbance of some light wavelengths by solutes dissolved in solution‚ while allowing other wavelengths to pass through (transmittance). The combination of the remaining wavelengths that pass through results in the colour of the solution. A colorimeter can be used to determine the amount of light at a particular wavelength that is absorbed/transmitted by a solution. Depending on the concentration
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Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate student’s writing of lab reports by providing this information in an editable file which can be sent to an instructor. Exercise
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