Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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What is it? Sickle cell anemia is a type of blood disorder that can be inherited from parents. This is when red blood cells that are meant to carry oxygen around the body become deformed. Normal red blood cells are round and disc shaped‚ however‚ when they become sickle red blood cells‚ the deform and change shape into a crescent-like shape or sickle shape. The sickle cells have a shorter life-span than a healthy red blood cell and are not replaced as quickly as normal; this leads to a shortage
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CE121: Construction Materials Laboratory Report No. 1 Particle Size Distribution Van Joseph E. Casalme 2010-14928‚ BS Civil Engineering Institute of Civil Engineering‚ College of Engineering University of the Philippines Diliman‚ Diliman Quezon City 1101 Submitted to: Engr. Christian R. Orozco ABSTRACT This test (ASTM C136-06) determines the grading of materials being used as aggregates using two parameters (coefficient of uniformity and gradation) from particle-size distribution
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Acid Base Titration Purpose: The purpose is to calculate the molarity of a NaOH solution by titrating the base with 5mL of standard HCl solution in each trial. By adding the base with unknown molarity to the acid with 0.10M the molarity of NaOH can be calculated. The base‚ NaOH‚ helps bring the pH of the acid‚ HCl‚ closer to seven‚ which neutralizes it. When using the buret the amount of NaOH used is able to be determined. Then by writing a balanced chemical equation and using the titration
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Respiration: How Cells Harvest Chemical Energy 1. A cell must work just to maintain its complex structure‚ for order is intrinsically unstable. Because of entropy (the second law of thermodynamics)‚ which states that any enclosed system tends toward disorganization‚ cells must constantly use energy to keep themselves in order. If the cell was to stop using energy and let itself go then it would eventually get so disorganized that it wouldn’t function properly. 2. With the help of enzymes‚ the cell systematically
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Bubble Inside a Bubble Materials • • • • • • • • Granulated sugar (we had our best results using Imperial Sugar and Dixie Crystals) Dish soap Water Tablespoon Scissors Pipette Cup Adult supervision Bubbles form because of a combination of water’s hydrogen bonds and the oily film you can see shimmer in the light. The oily film you see is actually two separate layers of soap attached to‚ and surrounding‚ hydrogen-bonded water. Solar Oven S’mores Materials • Pizza box • Two clear sheet protectors
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Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
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INTRODUCTION There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous
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simple mechanisms used to convert rotary motion into oscillating linear motion and vice-versa. The first of these is the slider-crank - a mechanism widely used in engines to convert the linear thrust of the pistons into useful rotary motion. In this lab we will measure the acceleration of the piston of a lawn mower engine at various speeds. The results exemplify a simple relation between speed and acceleration for kinematically restricted motions‚ which will discover. An adjustable slider-crank apparatus
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Epithelial tissue covers the whole surface of the body. It is made up of cells closely packed and ranged in one or more layers. This tissue is specialized to form the covering or lining of all internal and external body surfaces. Epithelial tissue can be divided into two groups depending on the number of layers of which it is composes. Epithelial tissue which is only one cell thick is known as simple epithelium. If it is two or more cells thick such as the skin‚ it is known as stratified epithelium.
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