Cell Biology Lab - Homework 1 Due to the week of Oct. 6-10th This is NOT a lab group project. Do the work individually. You may use any source other than your fellow students. Show your work‚ use your own words and cite all references. 1. (20 points) The HeLa S3 cancer cell line is considered to be “continuous” due to acquired mutations that enable the cells to evade senescence. This property is one of several hallmark characteristics of cancer cell lines. Describe three hallmark characteristics
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1) Hypertonic refers to _______. 1) _______ A) the concentration of solutes that a cell is bathed in B) a concentration of solutes that is less than the solutes inside the cell C) the concentration of solutes inside a cell D) a solution that will cause a cell to swell 2) What happens to facilitated diffusion when the protein carriers become saturated? 2) _______ A) ATP will be utilized for transport. B)
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Observations: When Malaria is present and infects red blood cells‚ parasites can infect cells carrying defective hemoglobin which may result in death. Allele frequency changes over time depending on the pressures or circumstances facing a particular population. African populations are especially impacted by both malaria and sickle cell anemia. Depending on the impacted population‚ allele frequency often shifts and well suited organisms are likely to survive and allele frequencies can increase
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Determination of an Electrochemical Series In electrochemistry‚ a voltaic cell is a specially prepared system in which an oxidation-reduction reaction occurs spontaneously. This spontaneous reaction produces an easily measured electrical potential which has a positive value. Voltaic cells have a variety of uses and you commonly refer to them as a “battery”. Half-cells are normally produced by placing a piece of metal into a solution containing a cation of the metal (e.g.‚ Cu metal in a solution
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affect the cell membrane‚ thus would then releases the purple pigments out of the vacuole which causes the leakage of the purplish liquid. Background Information The outermost layer is the cell wall‚ which is present only in plant cells and is made up of a carbohydrate called cellulose and also has other protein substances embedded within it. The cell wall is a rigid layer and gives structural stability to the cell and also limits the permeability of large substances into and out of the cell. Within
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01) The Cell Structure and function Abstract In this lab the structures of various cells were viewed through a compound microscope. The bacteria in yogurt was viewed a long with a slice of potato‚ and a thin skin of red onion. The potato was dyed with iodine while the red onion was dyed with methane blue. The students had to assemble the first three slides. Two more slides were provided these are the yeast and protozoa cells. Those two are also viewed
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Pre-Laboratory Questions 1. Yeast cells look like separate little spheres; they cannot walk or swim. Brainstorm methods by which yeast cells might communicate with each other. Record your list of possibilities in the space below. I. Signal Cascade II. Hormones III. Pheromones IV. Receptors embedded in the cell V. Growth near another cell Guided Activity: Table Charts Alpha – Type Culture Single Haploid Cells Budding Haploid cells Time 0 Field 1 3 3 Field 2 1 1 Field 3 8 2
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TITLE AND AUTHOR Lab 7 Analysis of purified Concanavalin A via:Hemagglutination INTRODUCTION The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. If ConA is active then agglutination will occur due to ConA’s free receptors being able to bind to the glucose residues on the sheep’s red blood cells. If ConA is not active then no agglutination will occur. To test the hemagglutination reaction‚ two types of ConA solutions
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Objectives: To study the effects of hypotonic‚ hypertonic and isotonic solutions on plant and animal cells. Materials and Apparatus: * Microscope * Electronic balance * Stirring rod * Small knife * Glass slide and cover * 250‚ 500 ml beakers * Distilled water * Cotton * Test tubes * Spatula * Onion * Ethanol (70%) * Filter paper * Table sugar (sucrose) * Table salt (NaCl) * Potato EXPERIMENT 1 PART 1 Procedure: 1. The epidermal
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the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel onto the wet membrane and another Whatman paper was placed on top of the. The cathode plate was placed and started the transfer. Then the membrane was placed in TBST containing 5% milk for 60 minute while shaking. The membrane was rinsed
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