Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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investigation I will be measuring the effects of temperature on the membrane permeability of beetroot. I will be measuring the amount of anthocyanin that will diffuse out of the beetroot. The way in which I will measure the anthocyanin is to check the light absorbency of the solution using a colorimeter. The higher the reading on colorimeter the more anthocyanin present in the solution To find out the permeability of the beetroot membrane I will firstly cut out cylinders of beetroot using a cork borer
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Under Microscope Lab Report A. The purpose of the investigation is to determine the time an onion cell spends in each part of the cell cycle. B. The significance of this investigation is to help us understand and know how long cells are undergoing mitosis. My personal interest is to know the phases of mitosis in cells in order to someday help find cures for cancer. C. The cell cycle is a chronological set of events‚ culminating in cell growth and division into two daughter cells. Some of the reasons
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Investigating the effects of temperature on cell membranes Independent variable: Temperature of beetroot Dependent variable: Absorbency of light Hypothesis: As temperature rises to its optimum temperature‚ the absorbency of light will increase because protein is an enzyme and will therefore be affected by temperature. This is because more beetroot dye will pass through the cell membrane and then into the distilled water. The deeper the colour of the beetroot dye‚ the higher the absorbency
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John Leone Lab Partner(s) Ethan Davis & Ryan Hanna Date: 12/10/14 Instructor: Dr. Chuckran Period: H Plants and Animal Cells Abstract: In this lab‚ you will find out how plant and animal cells are alike and how they are different. The onion elodea cells were both plants‚ so they had a cell wall. The cheek cells were animal cells‚ so they did not have a cell wall. Eukaryotic cells contain structures called organelles that carry out life processes. Eukaryotic cells can be classified by the types of organelles they contain
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1a. Aquaporins are membrane proteins that have fourteen various structures however the most common form is that of a homotetramer. The homotetramer is often found in the membrane and composed of four aquaporin membranes. The structural arrangement creates a fifth channel in the middle that allows for gas transportation. 1b. The direction of H2O molecular flow through a channel is determined by an osmotic gradient. 1c. One of the mechanisms that prevents proton channeling is due to the (ar/R) and
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Plasma membranes are selectively permeable. The purpose of this experiment is to reveal how a form of diffusion in a semipermeable membrane works by creating a real life simulation of a semipermeable plasma membrane. This is done by building an imitation of a caterpillar’s digestive tract using dialysis tubing and glassware. The first material in this experiment is a small beaker representing the head and crop of the caterpillar. This beaker is filled with both starch and the enzyme α-amylase. This
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Stress and Cellular Membranes Bio Lab SBI 4U1 Due: Sept 24th‚ 2010 For: Ms. Barton By: Ameema Saeed Introduction: (See attached lab) Purpose: (See attached lab) Hypotheses: Tap water will cause no damage to the membranes. The extreme temperatures of the water cooled to 0°C‚ and heated to 94-100°C will greatly damage the membranes. Extreme heat will be more damaging than extreme cold. The more concentrated the solvent‚ the more damage it will cause to the membranes‚ therefore the solvent
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“Cell Transport Lab” Hypothesis: I hypothesis that there will be a movement of molecules out of the egg‚ and will move into the water solution. Osmosis will occur within the egg‚ and in the process the molecules of the egg will diffuse. Time (hrs) | Observations | Circumference (cm) | * 0 | * Egg is of normal weight and size * No membrane is being shown‚ and is almost perfect | * 14 centimeters | * 24 | * Egg has gotten larger taking in some of the water * Shell is soft
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The cell‚ which is the smallest unit of life‚ is surrounded by a plasma membrane. The plasma membrane functions somewhat like a wall‚ as it keeps the internal contents from the external environment. Just like a wall‚ the membrane is also somewhat permeable‚ except that the membrane takes a much more active role in determining what is allowed in to the cell and what is kept out. The plasma membrane is a very thin structure‚ which has some very important tasks. One of the main tasks that it performs
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