Determining Standard Reduction Potentials‚ Equilibrium Constants and Investigating a Lead-Acid Electrolytic Cell Purpose Experimental Methods All procedures were followed according to the lab manual (experiment 10 – Electrochemistry Laboratory). Data and Observations Part A: Grams of FeSO4 used: 0.759 Voltage during voltmeter and battery check: 9.23V Table 1. Electrochemical Cells Data Part B: Table 2. Lead-Acid Battery Results and Calculations In order to make 5ml of
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Embryonic Kidney(HEK) 293 cells were transiently transfected with pEGFP-N1(Clontech‚ Palo Alto‚ CA‚ encoding enhanced GFP as an indicator of transfection) and pEGFP-LeIF plasmids cloned at previous studies(Ghafarifar and et al‚ in press) by Calcium phosphate and non- tranfected cells was used as negative control. briefly‚ Hek-293 cells were grown at 37°C in Dulbecco’s Modified Eagle Medium (DMEM‚ Gibco) supplemented with 10% Fetal Bovine Serum (FBS‚ Gibco) and 5% CO2. Log-phase cells were employed for transfection
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BIO 353 Cell Biology Study Questions for Exam #3 Fall‚ 2012 Cytoskeleton “The harder the conflict‚ the more glorious the triumph.” Thomas Paine... “Unless you really just screw up.” Bush 1. What are microfilaments? (He said know how to identify/draw these) Note: since it is multiple choice‚ I doubt we will have to know how to draw them. **There are also short essay questions and‚ in past classes‚ he has asked students to draw. I thought the final was ALL MULTIPLE CHOICE???********************************
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Cells‚ in a lot of ways‚ are like factories. All the parts of a cell work together to accomplish common goals‚ just like parts of a factory. Each part also has a specific function‚ to make getting the job done a lot easier. The floor of a factory is a lot like the cytoplasm in a cell. It kind of keeps everything where it needs to be‚ and helps direct organelles around the cell‚ much like the pathways on the floor around the machines. The cell membrane in a cell is very similar to the outside
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Cells of the body are organized into four fundamental tissues; epithelial‚ connective‚ muscle and nerve. Epithelial tissues line all surfaces of the body: the skin‚ cavities‚ ducts‚ vessels. They are named according to the cell shape on the free surface. Cells of a tissue are held together by the basement membrane and intercellular fibbers. These tissues receive their nutrition by diffusion‚ for they are without blood vessels. Simple squamous epithelium lines all blood and lymphatic vessel‚ including
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The Cell Cycle Mitosis: the process by which cells reproduce themselves‚ resulting in daughter cells that contain the same amount of genetic material as the parent cell. Interphase Prophase Metaphase Anaphase Telophase Cytokinesis Cell Division Occurs in a series of stages of phases Interphase occurs before mitosis begins Chromosomes are copied (# doubles) Chromosomes appear as threadlike coils (chromatin) at the start‚ but each chromosome and its copy(sister chromosome) change to sister
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solutions of each substance were sterilized by passing them through a 0.22 μm (Nupore Filtration Systems‚ UP‚ India) filter. The actively growing cell suspension was treated with elicitors and precursor indiviually on the day of inoculation. The effect of elicitors (ABA‚ SNP‚ SA‚ and MeJA) and precursor (Phenylalnine) on stimulation of anthocyanin in the cell suspension cultures of D. carota was studied at various concentrations viz. 10‚ 50‚ 100‚ 200 μM and phenylalanine viz. 0.5‚ 1 and 5 mM.The samples
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Cell Fractionation: Isolation of Mitochondria from Cauliflower and Determination of Specific Enzyme Kinetics Introduction Mitochondria is an organelle found in eukaryotic cells that play a role in biochemical processes such as respiration and energy production. Mitochondria even play an important role in apoptosis‚ or programmed cell death. This is achieved by disruption of electron transport‚ oxidative phosphorylation‚ and ATP production or even the release of proteins that trigger activation
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light microscope when you want to see the cell in the act of moving or dividing. 2. Prokaryotic cells don’t have organelles like eukaryotic cells do. Eukaryotic cells contain its DNA within its nucleus‚ while prokaryotic cells keep it within the nucleoid. Prokaryotic cells are also more minute than the eukaryotic cells. Also although they both contain ribosomes‚ they are composed differently. 3. The central vacuole (takes in water and chemicals)‚ cell wall (allows the plant to be strong enough
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Name: Ranessa Dockery I.D #: 27100017 Course: Immunohematology Lab Instructor: Sala Randall Date: January 22‚ 2013 Lab #: 1 Title: Cell Washing Aim: To demonstrate quality assurance techniques in performing cell washing. Principle: Cell washing is a common procedure performed in the laboratory. This is a series of careful steps taken to wash red blood cells normally three times intermittently with centrifugation and decanting (Harmening 2012). The procedure serves to
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