permeability of beetroot Hypothesis Organic solvent and high temperature will destroy the cell membrane and make it permeable to the red pigment. The higher the concentration of the alcohol‚ the greater will be this effect. Principle Beetroot is the material used in this experiment to demonstrate the effects of high temperature and chemicals on the permeability of the cell membranes. Beetroot contains a red pigment called betalain‚ which is located in the large central vacuole of the beetroot cells.
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Biology A - Assignment 1: Cell Membranes and Temperature Background Theory Beetroot contains betalain pigments within it’s vacuole‚ which gives it it’s purple-red colour‚ it’s this pigment which leaks out as the beetroot is heated and the cells membrane begins to break down. The practicals aim is to show the effect of temperature on the cell membrane by observing the colour change of the water the beetroot was placed in. The cell membrane is important as it controls the substances enters and
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effect of variation in temperature on the permeability of cell membranes using fresh beetroot Aims In this investigation‚ you will subject fresh‚ washed beetroot discs to different temperatures to investigate the effect of temperature on the permeability of the plasma membrane. Overview The beetroot discs will be placed in distilled water at different temperatures. You will compare the intensity of the beetroot pigment that has leaked out from the cells subjectively and possibly objectively using
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BRIEF INTRODUCTION: Beetroot cells contain a red pigment stored in the cell vacuole in the membrane to prevent leaking out from the cell. In this experiment we used several solvents to calculate which one made the cell release more pigment. RESULTS: A table showing the results of the intensity of the color and the absorbance of the liquids with the different solvents. Solvent Results from colorimeter 0.001 Acid (Hydrochloric) 0.358 Ethanol 0.132 Hot water 0.984 Cold water-distilled
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fatty acid composition. This means that at temperatures below this critical point no dye in the beetroot cell would be able to leak from the cell and would not change the colour of the surrounding water. The phospholipids also undergo phase changes at higher temperatures causing the membrane to be more permeable thus allowing the red pigment to move more freely into the water surrounding the beetroot. Sources of Background Information New Understanding Biology for Advanced Level Fourth addition
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Apparatus and materials: 1 tube mouth rinse(4 brands)‚ 3 cylinders beetroot‚ 1 pc razor blade‚ 1 pc white tile‚ 10 pcs test tubes‚ 2 pcs test tube racks ‚ 1pc 10-mL measuring cylinder‚ 5 pcs labels ‚ 1 pc forceps‚ 1 pc 500-mL beaker Aim : To study the effect of different brands of mouth rinse on the permeability of the membranes of beetroot tissue Problem: To compare the denaturation of membrane of cells in the effect of mouth rinse of different brands and find out which brand of mouth rinse
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effect of temperature on cell membrane structures Procedure SAFETY: Always carry scalpels clasped to a tile and with the tip pointing away from you. Beetroot cells contain pigments called betalains that give the tissue its dark purple-red colour. The pigment is contained in the cell vacuole. Investigation a. Collect 3 or 4 beetroot cores from the beaker provided. Cut each core into 2 cm sections until you have enough for one core for each temperature of water bath that you will be using
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making the membrane fragile. In beetroot when the membrane is damaged‚ the red pigment it contains is released. Variables
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variable: Temperature of beetroot Dependent variable: Absorbency of light Hypothesis: As temperature rises to its optimum temperature‚ the absorbency of light will increase because protein is an enzyme and will therefore be affected by temperature. This is because more beetroot dye will pass through the cell membrane and then into the distilled water. The deeper the colour of the beetroot dye‚ the higher the absorbency of light. Beetroot vacuoles have red pigments which can’t pass through the
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betacyanin‚ a red pigment‚ located in the large internal membrane vacuoles. When the membrane is damaged‚ the pigment can cross the vacuole membrane and cell membrane. Since pigment is water soluble and not lipid soluble‚ it remains in the vacuole when the cells are healthy. If the integrity of a membrane is disrupted‚ however‚ the contents of the vacuole will spill out into the surrounding environment. This usually means the cell that made the vacuole is dead. Variables. Age of beetroot. The age of
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