test is a sensitive chemical test for the presence of carbohydrates‚ based on the dehydration of the carbohydrate by sulphuric acid to produce an aldehyde‚ which condenses with two molecules of phenol‚ resulting in a red- or purple-colored compound. Benedict ’s test allows for the detection of the presence of reducing sugars (sugars with a free aldehyde or ketone group). All monosaccharides are reducing sugars since all of them have active carbonyl group. Some disaccharides that are exposed to a carbonyl
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indicating the presence of starch. The Iodine solution traps itself in the helical structure of the polysaccharide to form dark blue colour. 3. To test for the presence of glucose‚ Benedict’s solution was used to test for reducing sugars. Benedict’s reagent is clear blue due to the copper
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solution. We then remove the bag out of the beaker‚ and use two test tube which label BAG‚ BEAKER to perform a Benedict’s test. We place solution in the bag in the BAG tube‚ and solution in the beaker in the BEAKER tube and add a drop of Benedict’s reagent to each tube‚ then heat each test tube and observe a color change in each tube. These experiments show that movement of water of chicken egg cell and Elodea cell is “osmosis” and movement of molecules in dialysis bag and beaker is “diffusion”.
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Analysis: Lab 1 Identifying Carbohydrates There were many ranges of color changes in the Benedicts test and Iodine stain. For the Benedicts test‚ this oxidation/reduction response changes the arrangement of the reducing sugar creating a colored precipitate. The change in color is correlated to the first concentration of the reducing sugar. For the iodine test‚ the three dimensional structure of various polysaccharides permits them to react with the iodine stain to produce a certain color. As far
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The Effect of Osmosis on Potatoes/ Diffusion of Molecules through Benedicts Test of Dialysis dubing Introduction The focus of the lab on September 16‚ 2013 was Diffusion and Osmosis. Osmosis is a process in which the molecules of a solvent diffuse from an area of high concentration to an area of lower concentration‚ through a semipermeable membrane. Small solute molecules and water molecules can move freely through a selectively permeable membrane‚ but large molecules may pass through more
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reaction. The second TLC is to monitor the progress of the recrystallization. In the second part of the experiment‚ the goal is to determine which test tubes with unknown A and B contains glucose or sucrose. Benedict ’s test is a way to determine the presence of aldehydes in a compound. The benedict ’s solution contains copper (II) sulfate and citrate which together can form a complex. This complex acts as an oxidant to oxidize aldehydes to carboxylic acids. The copper (II) sulfate is reduced and precipitate
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The objective of this macromolecules lab was to identify the presence any of the major macromolecules in various every day food items. The three macromolecules that this lab was carried out for were carbohydrates‚ lipids‚ and proteins. There were five different experiments conducted and each of those experiments had one factor in common‚ they all had the same controls. The controls in this lab activity were already set for the lab activity. The controls were the distilled water and the baking soda
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translucent or opaque spot will appear. Materials: * 8 test tubes * test tube rack * test tube holder * glass stirring rod * sharpie marker * how water bath * iodine solution * brown paper bag * Biuret reagent * benedicts solution * 20 ml each: 1. honey solution 2. egg white and water solution 3. gelatin and water solution 4. melted butter and water solution 5. apple juice and water solution 6. distilled water 7. unknown solution
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Materials: 2 safety goggles 1 eye dropper Heat source 2 beakers 4 test tubes 1 test tube clamp 1 test tube rack 1 glass stirring rod 3 teaspoons canned whole-kernel corn 10 ml fresh‚ whole‚pasteurized milk 1 small biuret reagent 1 small benedicts solution 1 small bottle iodine 1 small bottle sudan 4 1 blender Procedures: 1)Heat water in a graduated cylinder . 2)Blend your corn in the blender and add as much water so that it turns into a liquid. 3)Pour 10 ml of fresh milk
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Important Molecules‚ we are able to perform tests such as the Benedicts Test for Reducing Sugars‚ Iodine Test‚ Biuret Test‚ Sudan IV Test‚ and lastly the Grease-Spot Test that detect the presence of biologically important carbohydrates‚ protein lipids and nucleic acids. Most known compounds in living organism are if fact carbohydrates‚ lipids‚ proteins‚ and nucleic acids. Each of these macromolecules are tested differently. The Benedicts test identifies reducing sugars based on their ability to reduce
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