5. Mash the bag with the lysis buffer for 2 minutes. CAUTION: Mash the mixture carefully; the fewer bubbles created the better the results. 6. Place the funnel in the flask and place the coffee filter in the funnel. 7. Cut one of the bottom corners of the baggie off and slowly squeeze the strawberry liquid and the pulp into the funnel (in this case coffee filter). 8.
Premium Cell DNA Gene
types of Mixtures? Define:-solutions -suspensions -colloids (Draw and compare.) Methods of separation 1. Filtration 2. Distillation (simple and fractional) 3. Paper chromatography 4. Solvent extraction 5. Separating funnel 6. Evaporation 7. Crystallization 8. Sublimation Draw and explain each. What is a mixture??? A mixture is a combination of two or more substances that are not chemically united and do not exist in fixed proportions to each other
Premium Mixture Solution Liquid
The Effect of Light Intensity on Photosynthesis and Transpiration Inna V. Raymundo BOTANY I Section V-6L August 29‚ 2012 ¹A scientific paper submitted in partial fulfillment of the requirements in Botany I laboratory under Dr. Nonnie Bautista‚ 1st sem‚ 2012-2013. ABSTRACT Photosynthesis and transpiration both play important roles in plants. But there are factors which affect the rate of photosynthesis and transpiration. The external factor that was identified is the
Premium Plant Photosynthesis Pigment
Introduction Extraction is a purification technique used in organic chemistry to separate compounds from a mixture of two or more compounds. There are three different extraction techniques: liquid-liquid extraction‚ solid-liquid extraction and chemically active extraction. All three types of extraction follow the same principle. Organic molecules dissolve in organic solvents and polar molecules dissolve in aqueous solvents. This phenomenon is observed because of the intermolecular forces between
Premium Solubility Solvent Chemistry
kindergarten. The indoor water play is located in the basement. Their names are Anna‚ Dean and Amy. The water play area had a huge container of water. There were about ten different water play materials for the children to manipulate‚ for example‚ funnels‚ hose‚ cups‚ jugs‚ containers and animals. The teacher-in-charge would fill the basin with water before every play and after which‚ she would approach the water play area every five minutes and facilitate the children’s play. Observations Anna
Free Natural environment Environment Environmentalism
STEREOCHEMISTRY: ADDITION OF BROMINE TO trans-CINNAMIC ACID Required Prelab Readings: McMurry Chapter 5‚ Sections 8.2 & 21.2 Morhig‚ Sections 7.1 and 7.3. Previous techniques that you must know and be able to perform: Suction Filtration and Melting Point This experiment is designed to demonstrate two concepts. First‚ it will provide a demonstration of how chemists can use chemical reactions to understand reaction mechanisms. Second‚ is the concept of multi-step synthesis. You will
Premium Chemistry Chemical reaction
Producing Isoamyl Acetate from Isoamyl Alcohol and Acetic Anhydride through Esterification Racquel Erica D.G. Isada‚ Jannah B. Juan*‚ Moses Isaiah L. Koh and Hedder A. Lim Department of Biology‚ College of Science University of Santo Tomas‚ España Street‚ Manila 1051 Date Submitted: August 13‚ 2014 Abstract: In this experiment‚ the synthesis of Isoamyl acetate from Isoamyl alcohol and Acetic anhydride was prepared with the process of Esterification. By using the reflux technique‚ extraction
Premium Ester Carboxylic acid Acetic anhydride
Synopsis The objective of this experiment is to determine the percentage by mass of calcium carbonate‚CaCO3 in toothpaste using back titration technique. A known weight portion of toothpaste is obtained to react with known volume and concentration of standard acid solution. After completing the reaction‚ the resulting solution containing excess acid is back titrated with known volume and concentration of standard base solution. Determination of excess acid after reaction allow us to calculate the
Premium Titration
www.hbrreprints.org In many companies‚ sales forces and marketers feud like Capulets and Montagues— with disastrous results. Here’s how to get them to lay down their swords. Ending the War Between Sales and Marketing by Philip Kotler‚ Neil Rackham‚ and Suj Krishnaswamy Included with this full-text Harvard Business Review article: 1 Article Summary The Idea in Brief—the core idea The Idea in Practice—putting the idea to work 3 Ending the War Between Sales and Marketing 14 Further Reading A list
Premium Marketing Sales
After mixture dissolves‚ heat for 8 to 10 minutes. Crystallize the Acetylsalicylic acid by cooling the mixture. Then add 3.0mL of water and stir. Set up a Hirsh funnel for vacuum filtration. Add 1mL of cold water to help transfer all of crystallized substance. Rinse crystals with water once all collected in funnel. Let crystals dry in funnel. Let finish drying on a watch glass for the week. After a week take the product‚ and weigh it in a pre-weighed beaker. Calculate the percent yield. Test for
Free Aspirin Carboxylic acid Acetic anhydride